Rabbit anti-Human PLZF Monoclonal Antibody for Intracellular Flow Cytometry - Q05516

Core Product Specifications and Parameters

Biological Background: PLZF Function and Localization

• Zinc finger and BTB domain-containing protein 16 (ZBTB16), also known as promyelocytic leukemia zinc finger protein (PLZF) or zinc finger protein 145 (ZNF145), is a transcription factor characterized by multiple zinc finger motifs.
• Functions as a transcriptional repressor by recruiting histone deacetylases to target gene promoters (PubMed:10688654, PubMed:24359566).
  Related references: PMID:10688654 PMID:24359566
• Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex, mediating ubiquitination and proteasomal degradation of target proteins (PubMed:14528312).
  Related references: PMID:14528312
• Plays a role in myeloid maturation and the development or maintenance of differentiated tissues.
• Localizes to the nucleus, notably in nuclear bodies, consistent with its role in transcriptional regulation.
• Expressed in hematopoietic tissues including bone marrow and early myeloid cells, and also found in ovary, kidney, and lung.
• Undergoes phosphorylation and is involved in chromosomal rearrangements associated with acute promyelocytic leukemia; recognized as a proto-oncogene.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, follow standard fixation and permeabilization protocols appropriate for nuclear antigens.
• The antibody was generated against a recombinant protein corresponding to amino acids 6-137 of human PLZF, an N-terminal region; epitope mapping may be useful for specific applications.
• Due to human cross-reactivity, validate the antibody in species-specific contexts if non-human samples are to be analyzed.
• Titration experiments are recommended to determine the optimal antibody concentration for your specific cell type and experimental conditions.
• Ensure appropriate isotype controls are used for accurate gating in flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic development. This anti-PLZF antibody is part of our tailored portfolio for transcriptional regulation and leukemia research, enabling reliable performance across life science applications. From concept to clinic, our integrated supply and technical services simplify your procurement process.