Rabbit anti-Human PLZF Monoclonal Antibody for Intracellular Flow Cytometry - Q05516

Core Product Specifications and Parameters

Biological Background: PLZF Function and Localization

• Zinc finger and BTB domain-containing protein 16 (PLZF), also known as ZNF145, is encoded by the ZBTB16 gene. It belongs to the BTB/POZ domain-containing zinc finger transcription factor family.
• Acts as a transcriptional repressor, mediated at least in part through the recruitment of histone deacetylases to target promoters. Related references: PMID:10688654, PMID:24359566
• Plays a role in myeloid maturation and the development and/or maintenance of other differentiated tissues.
• Functions as a probable substrate-recognition component of an E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins. Related references: PMID:14528312
• Localizes to the nucleus, including nuclear bodies, consistent with its function in transcriptional regulation.
• Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines, and peripheral blood mononuclear cells. It is also found in the ovary, and at lower levels in the kidney and lung.
• Contains nine C2H2-type zinc fingers and a BTB/POZ domain; known to undergo alternative splicing and is associated with chromosomal rearrangements in acute promyelocytic leukemia.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry applications, use appropriate fixation and permeabilization protocols to enable antibody access to nuclear PLZF.
• The immunogen spans amino acids 6–137, targeting the N-terminal region of PLZF; consider this when designing epitope mapping or competitive assays.
• This antibody is validated for human samples; cross-reactivity with other species has not been reported and should be independently validated if needed.
• Optimize antibody dilution and staining conditions in your specific cell type and experimental setup to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this anti-PLZF antibody ideally suited for research in transcriptional regulation and myeloid cell development. We provide validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline your assay development. For rare or custom targets, our sourcing expertise ensures reliable access to the critical raw materials your diagnostic projects demand.