Rabbit anti-Human Phospho-PI3K p85α (Y467)/p85β (Y464)/p55γ (Y199) Monoclonal Antibody for Intracellular Flow Cytometry - P27986 / O00459 / Q92569

Core Product Specifications and Parameters

Biological Background: PI3K Regulatory Subunits (p85α/p85β/p55γ) Function and Localization

• The phosphatidylinositol 3-kinase (PI3K) regulatory subunit p85α (encoded by PIK3R1) is an adapter protein that plays a critical role in mediating the activation of the PI3K/AKT signaling pathway. It contains SH2 and SH3 domains that bind to phosphorylated tyrosine residues on activated receptor tyrosine kinases and adaptor proteins, recruiting the p110 catalytic subunit to the plasma membrane to produce PIP3 and propagate downstream signals. Related references: PMID:17626883, PMID:19805105, PMID:7518429
• This antibody is designed to recognize the phosphorylated forms of three PI3K regulatory subunits: p85α (PIK3R1), p85β (PIK3R2), and p55γ (PIK3R3), specifically at the conserved tyrosine phosphorylation sites Y467 (p85α), Y464 (p85β), and Y199 (p55γ). These phosphorylation events are associated with activation of PI3K signaling downstream of growth factor receptors.
• p85α is necessary for insulin-stimulated glucose uptake and glycogen synthesis in insulin-sensitive tissues and plays a role in signaling in response to various growth factor receptors (e.g., FGFR, KIT, PDGFR). It also modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2, thereby contributing to glucose tolerance improvement. Related references: PMID:20348923
• Subcellularly, p85α is predominantly localized to the cytoplasm, where it interacts with membrane-bound receptors upon stimulation.
• Isoform 2 of p85α shows tissue-specific expression, being present in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. At the protein level, isoforms 2 and 4 are detected in skeletal muscle.
• Key post-translational modifications include phosphorylation on tyrosine and serine/threonine residues, acetylation, and ubiquitination, which regulate its stability and interaction with signaling partners. p85α is also involved in host-virus interactions.

Experimental Guidance and Technical Tips

• For intracellular phospho-flow cytometry, consider using fixation/permeabilization protocols optimized for phospho-epitope preservation. Pre-incubation with phosphatase inhibitors may improve signal stability.
• Validate staining with appropriate positive and negative controls (e.g., untreated vs. growth factor-stimulated human cells) to confirm phospho-specificity.
• As this antibody is directly conjugated to , perform titration experiments to determine the optimal concentration for your cell type and experimental conditions.
• Ensure that the chosen fluorophore is compatible with your flow cytometry panel and instrument configuration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This phospho-PI3K regulatory subunit antibody supports research into PI3K/AKT signaling, metabolic regulation, and cancer biology. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and facilitate rare target raw-material sourcing to accelerate your IVD development pipeline.