Rabbit anti-Human Phospho-PI3K p85α (Y467)/p85β (Y464)/p55γ (Y199) Monoclonal Antibody for Flow Cytometry (Intracellular) - P27986 / O00459 / Q92569

Core Product Specifications and Parameters

Biological Background: PI3K Regulatory Subunits (p85α/p85β/p55γ) Function and Localization

• Phosphatidylinositol 3-kinase regulatory subunit alpha (PIK3R1) encodes the p85α regulatory subunit, which exists in multiple isoforms via alternative splicing, including isoform 2 expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle.
• Contains one SH3 domain and two SH2 domains that mediate binding to phosphorylated tyrosine residues on activated receptor tyrosine kinases and adaptor proteins, linking p110 catalytic subunits to the plasma membrane.
• Functions as a critical adapter in PI3K/AKT signaling, necessary for insulin-stimulated glucose uptake and glycogen synthesis; also acts downstream of FGFR, KIT, PDGFRA/B, and ITGB2. Related references: PMID:17626883, PMID:19805105, PMID:7518429
• Modulates endoplasmic reticulum stress response by promoting nuclear translocation of XBP1 isoform 2, improving hepatic glucose tolerance under metabolic overload. Related references: PMID:20348923
• The p85β (PIK3R2) and p55γ (PIK3R3) regulatory subunits share structural homology with p85α and contain analogous phosphorylation sites, though their tissue distribution and specific functions may vary.
• Keywords from UniProt include Phosphoprotein, SH2 domain, SH3 domain, Alternative splicing, Cytoplasm, and Ubl conjugation; the protein is subject to acetylation and ubiquitination.
• Subcellular location is primarily cytoplasmic, where it interacts with membrane-bound receptors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to Y467 of human PI3K p85α; cross-reactivity with homologous phosphorylated sites in p85β (Y464) and p55γ (Y199) is intended based on sequence conservation, but sequence alignment should be verified for off-target considerations.
• For intracellular flow cytometry, optimize permeabilization and fixation protocols to preserve phospho-epitopes; use appropriate phospho-specific controls and stimulation conditions (e.g., serum starvation followed by growth factor treatment) to confirm signal specificity.
• Since the antibody is directly conjugated to , no secondary antibody is needed, reducing background; include appropriate compensation controls when multiplexing with other fluorophores.
• Consider validating in relevant human cell lines known to express PI3K subunits and phosphorylate these sites upon activation of insulin or growth factor receptors.

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CamelBio serves as an integrated one-stop sourcing platform, providing diagnostic manufacturers and research laboratories with premium IVD raw materials including the phospho-specific PI3K regulatory subunit antibody detailed above. We support bulk ordering of validated antibody pairs, purified monoclonal antibodies, and ancillary reagents to streamline your assay development. Our expert team can also assist in sourcing rare targets or customized preparations, ensuring a reliable supply chain from early-stage research to clinical application.