Rabbit anti-Human Perforin mAb for FC (intra) - P14222

Core Product Specifications and Parameters

Biological Background: Perforin-1 Function and Localization

• Perforin-1 (PRF1, also known as cytolysin or lymphocyte pore-forming protein PFP) is a 61 kDa pore-forming glycoprotein that plays a key role in granzyme-mediated programmed cell death and immune defense against virus-infected and neoplastic cells. Related references: PMID:20889983, PMID:21037563, PMID:24558045
• It is essential for killing non-self target cells, such as in transplant rejection and certain autoimmune diseases. Related references: PMID:9058810
• In its calcium-bound form, Perforin inserts into the target cell membrane, oligomerizes, and forms large transmembrane pores, causing cytolysis and facilitating apoptosis. Related references: PMID:20889983, PMID:21037563
• Perforin mediates the uptake of cytotoxic granzymes into target cells, promoting efficient delivery of cationic cargo, while neutral or anionic proteins are transferred poorly. Related references: PMID:20038786, PMID:20225066, PMID:24558045
• Subcellularly, Perforin localizes to cytolytic granules, is secreted, and can be found in the endosome lumen and on the cell membrane. It contains EGF-like domains, disulfide bonds, and is a transmembrane beta-strand protein.
• Clinically, mutations in PRF1 are associated with familial hemophagocytic lymphohistiocytosis (FHL), a severe hyperinflammatory disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to nearly full-length mature Perforin (aa 21–555); the resulting antibody recognizes native and intracellular forms.
• conjugation enables direct detection by intracellular flow cytometry without a secondary reagent; we recommend optimizing fixation and permeabilization protocols for your specific cell types.
• The predicted molecular weight is 61 kDa; however, glycosylation and other modifications may result in additional bands or shifts in certain sample systems—validate in the relevant biological context.
• For best performance, titrate the antibody in your assay to determine the optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. This Rabbit anti-Human Perforin mAb supports intracellular flow cytometry applications, directly addressing immune function and cytotoxicity research needs. From concept to clinic, our technical consulting and supply capabilities help streamline your development pipeline.