Rabbit anti-Human PD-L1/CD274 Monoclonal Antibody for FC - Q9NZQ7

Core Product Specifications and Parameters

Biological Background: PD-L1/CD274 Function and Localization

• PD-L1 (Programmed cell death 1 ligand 1, also known as B7-H1) is a member of the immunoglobulin superfamily and functions as a ligand for the inhibitory receptor PD-1.
• It plays a critical role in maintaining immune tolerance by modulating T-cell activation thresholds and limiting effector responses (PubMed:11015443, PubMed:28813410, PubMed:28813417). Related references: PMID:11015443 PMID:28813410 PMID:28813417
• Through a yet unidentified activating receptor, PD-L1 may costimulate T-cell subsets that predominantly produce interleukin-10 (IL-10) (PubMed:10581077). Related references: PMID:10581077
• In response to hypoxia, PD-L1 can translocate to the nucleus via interaction with phosphorylated STAT3, acting as a transcription coactivator to promote GSDMC expression and pyroptosis (PubMed:32929201). Related references: PMID:32929201
• The PD-1/PD-L1 pathway is widely exploited by tumors to inhibit cytotoxic T lymphocytes and evade anti-tumor immunity, making it a key target for cancer immunotherapy (PubMed:28813410, PubMed:28813417). Related references: PMID:28813410 PMID:28813417
• Subcellularly, PD-L1 localizes to the cell membrane, early and recycling endosome membranes, and can be secreted or found in the nucleus.
• Tissue expression is highest in heart, skeletal muscle, placenta, and lung, with broader expression in activated immune cells such as T-cells, B-cells, dendritic cells, and monocytes.

Experimental Guidance and Technical Tips

• The antibody is conjugated to , making it directly suitable for flow cytometry (FC) without the need for secondary detection reagents.
• When establishing an FC staining protocol, consider titrating the antibody to determine the optimal concentration for your specific cell type.
• Validate the antibody in a relevant sample system, such as PD-L1-expressing tumor cell lines or activated human peripheral blood mononuclear cells (PBMCs).
• As PD-L1 can be upregulated upon activation, ensure appropriate stimulation conditions if analyzing primary immune cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutes, offering one-stop access to high-quality IVD raw materials—from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For immune checkpoint targets like PD-L1, our integrated supply chain and technical consultation support your assay development from concept to clinic, ensuring reliable sourcing for both routine and rare target needs.