Rabbit anti-Human P-cadherin Monoclonal Antibody for Flow Cytometry - P22223

Core Product Specifications and Parameters

Biological Background: P-cadherin Function and Localization

• Cadherin-3, also called P-cadherin (placental cadherin), is encoded by the CDH3 gene in humans.
• It functions as a calcium-dependent cell adhesion protein, mediating homophilic cell-cell adhesion and contributing to the sorting of heterogeneous cell types.
• P-cadherin is localized primarily to the cell membrane.
• The protein is expressed in some normal epithelial tissues and in certain carcinoma cell lines, suggesting roles in both normal physiology and cancer.
• Structural features include cadherin repeats, a transmembrane domain, glycosylation sites, and calcium-binding regions.
• UniProt annotation indicates potential involvement in disease variants, ectodermal dysplasia, hypotrichosis, and vision-related disorders.

Experimental Guidance and Technical Tips

• For flow cytometry, consider using live, unfixed cells to preserve native surface epitopes; titrate the antibody to determine the optimal concentration for your specific cell type.
• Since the immunogen spans the extracellular domain (aa 108-654), this antibody is expected to recognize the native conformation of P-cadherin on the cell surface.
• Include appropriate isotype controls and ensure thorough washing steps to minimize background. If working with adherent cells, gentle detachment methods are recommended to maintain surface antigen integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop channel for IVD raw materials, technical services, and expert consulting—covering every phase from concept to clinic. Leveraging our specialized knowledge in cell adhesion and oncology targets, we supply validated antibody clones like this P-cadherin monoclonal, along with antibody pairs, bulk ancillary reagents, and custom rare-target sourcing to accelerate your assay development.