Rabbit anti-Human OPN1LW/OPN1MW Monoclonal Antibody for Flow Cytometry - P04000 / P04001

Core Product Specifications and Parameters

Biological Background: OPN1LW/OPN1MW Function and Localization

• OPN1LW functions as a G(i)-coupled photoreceptor that, upon absorption of red light, isomerizes its chromophore 11-cis-retinal to all-trans-retinal, triggering a G protein-mediated cascade that decreases intracellular cAMP and ultimately mediates red-light visual perception. (PubMed:2937147, PubMed:6140680, PubMed:12051694, PubMed:8213841, PubMed:8666378) Related references: PMID:2937147 PMID:6140680 PMID:12051694 PMID:8213841 PMID:8666378

• The protein localizes to the membrane of photoreceptor outer segments in cone cells, where it is positioned for efficient photon capture.

• OPN1LW is the red-sensitive pigment in cone cells; the closely related OPN1MW (green-sensitive) and OPN1SW (blue-sensitive) complete the set of cone opsins required for trichromatic color vision.

• Structurally, OPN1LW is a seven-transmembrane GPCR with characteristic disulfide bonds, glycosylation, and phosphorylation sites, and it requires 11-cis-retinal as a chromophore.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-48) of OPN1LW/OPN1MW, suggesting the antibody may recognize native membrane-bound opsins, making it suitable for surface staining in flow cytometry without permeabilization.

• For FC assays, titrate the antibody using a relevant positive control (e.g., OPN1LW/OPN1MW-expressing cells) and include an appropriate isotype control to assess specificity.

• Protect the antibody from light during handling and storage to maintain the stability of the conjugate.

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CamelBio offers comprehensive IVD raw-material sourcing, from validated antibodies like this OPN1LW/OPN1MW flow cytometry antibody to ancillary reagents and custom services. Our expertise in cone opsin research tools supports diagnostic and research laboratories seeking reliable components for visual science applications. Contact us to explore bulk supply and technical consultation options.