Rabbit anti-Human OPN1LW/OPN1MW mAb for Flow Cytometry - P04000 / P04001

Core Product Specifications and Parameters

Biological Background: OPN1LW/OPN1MW Function and Localization

• OPN1LW (red cone photoreceptor pigment) is a G protein-coupled receptor that activates G(i) proteins to decrease intracellular cAMP levels in response to red light. Related references: PMID:2937147, PMID:6140680
• Upon photon absorption, the covalently bound 11-cis-retinal isomerizes to all-trans-retinal, inducing a conformational change that triggers the phototransduction cascade, ultimately mediating red color vision. Related references: PMID:12051694, PMID:8213841, PMID:8666378
• The gene is part of the visual pigment gene cluster on the X chromosome, adjacent to the green-sensitive OPN1MW gene; variations in these genes are linked to color vision deficiencies.
• OPN1LW localizes to the membrane of cone cell outer segments, specifically in the photoreceptor outer segment membrane.
• Keywords from UniProt indicate it is a phosphoprotein, glycoprotein, and contains a disulfide bond, with seven transmembrane helices typical of GPCRs.
• Tissue specificity: OPN1LW is expressed exclusively in cone photoreceptor cells of the retina, along with OPN1MW and OPN1SW (blue cone opsin).

Experimental Guidance and Technical Tips

• For flow cytometry, use a directly conjugated antibody to minimize background; is compatible with standard red laser lines (e.g., 561 nm) and common filter sets.
• Since the immunogen corresponds to the N-terminal region (aa1-48) shared with OPN1MW, this antibody is expected to detect both red and green opsins; confirm specificity in your sample system.
• The antibody is validated on human cells; cross-reactivity with other species is not guaranteed and should be tested empirically.
• Consider titrating the antibody to determine the optimal concentration for your FC assay, as staining intensity may vary with sample type and cell treatment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for vision-related research and diagnostic development. Whether you need matched antibody pairs for color vision disorder testing or custom-designed immunoreagents, we streamline procurement from discovery to clinical application. Contact us to source rare-target reagents like this anti-OPN1LW/OPN1MW mAb in bulk.