Rabbit anti-Human NT5E/CD73 Monoclonal Antibody for FC - P21589

Core Product Specifications and Parameters

Biological Background: NT5E/CD73 Function and Localization

• NT5E/CD73 is an ecto-5'-nucleotidase that catalyzes the hydrolysis of AMP to adenosine, playing a key role in purinergic signaling and adenosine generation.
• Shows substrate specificity for ribonucleotide monophosphates over deoxyribose forms, with activity against IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. Related references: PMID:21933152, PMID:22997138, PMID:23142347, PMID:34403084
• It is anchored to the cell surface via a GPI moiety, localizing exclusively to the cell membrane.
• The active site requires zinc binding, and the protein forms disulfide bonds important for structural stability.
• Multiple isoforms are produced through alternative splicing, potentially modulating enzymatic activity or localization.
• Post-translational modifications include N-linked glycosylation, which may affect protein folding and function.
• Genetic variants in NT5E are associated with calcification of joints and arteries (CALJA) and other pathological conditions, as indicated by the "Disease variant" keyword.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein containing amino acids 27–547 of human NT5E/CD73, corresponding to the entire extracellular domain. This design targets the native, surface-exposed epitope.
• For flow cytometry (FC), titrate the antibody to establish the optimal dilution for your cell type and staining protocol. Include an isotype control and, if possible, a positive control cell line known to express CD73.
• Given that CD73 is GPI-anchored, consider the potential for shedding or release into the supernatant under certain conditions; include appropriate controls to ensure specific surface staining.
• Since this antibody is directly conjugated to , it eliminates the need for a secondary antibody, simplifying the protocol and reducing background.
• As with any antibody, validation in the relevant biological context is recommended, especially when investigating rare cell populations or low expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing a comprehensive range of validated IVD raw materials, including this high-quality anti-NT5E/CD73 monoclonal antibody. Whether you need bulk quantities, customized antibody pairs, or ancillary reagents for nucleotide metabolism or immuno-oncology applications, CamelBio streamlines procurement from concept to clinic.