Rabbit anti-Human NG2 Monoclonal Antibody for FC - Q6UVK1

Core Product Specifications and Parameters

Biological Background: NG2 Function and Localization

• Identified as chondroitin sulfate proteoglycan 4 (CSPG4), also designated NG2, MCSP, MCSPG, MSK16, CSPG4A, HMW-MAA, or MEL-CSPG.
• Functions in cell proliferation, migration, and endothelial motility during microvascular morphogenesis; may also inhibit neurite outgrowth and growth cone collapse.
• Acts as a cell surface receptor for collagen alpha 2(VI) and binds growth factors and extracellular matrix proteases, modulating their activity.
• Regulates MMP16-dependent type I collagen degradation and invasion, and modulates plasminogen activation and angiostatin inhibition, contributing to melanoma cell invasion.
• Serves as a signal transducer by recruiting scaffolding and signaling proteins via its cytoplasmic C-terminus, activating Rho GTPases for retraction fiber formation and cell polarization.
• Promotes alpha-4, beta-1 integrin-mediated adhesion and spreading through a CDC42-ACK1-BCAR1 cascade, and may activate FAK and ERK1/ERK2 pathways.
• Localizes predominantly to the cell membrane, apical membrane, and lamellipodium membrane, consistent with its roles in adhesion and migration.
• Detected in fibroblasts, placenta, and malignant melanoma cells, reflecting its involvement in tissue remodeling and tumor biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1583–2224) of human NG2; verify its accessibility in your target cell type, as the epitope may be intracellular if the protein is not permeabilized.
• Optimized for flow cytometry (FC); titrate the antibody to determine the optimal working dilution for your specific cell line and staining protocol.
• Since the antibody is directly conjugated to , it can be used without a secondary detection step, reducing background and protocol time.
• Validate the antibody in a positive control cell line known to express NG2, such as human melanoma cells (e.g., A375), and include appropriate isotype controls.
• For intracellular staining, consider a fixation/permeabilization step; the large extracellular domain may require optimization of permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high-quality IVD raw materials. This NG2/CSPG4 antibody supports flow cytometry applications in oncology and angiogenesis research. Explore our portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents for your assay development needs.