Rabbit anti-Human NG2 Monoclonal Antibody for FC - Q6UVK1

Core Product Specifications and Parameters

Biological Background: Chondroitin sulfate proteoglycan 4 (NG2) Function and Localization

• Chondroitin sulfate proteoglycan 4 (CSPG4), also known as NG2, MCSP, MCSPG, MSK16, CSPG4A, HMW-MAA, or MEL-CSPG, is a transmembrane proteoglycan expressed on various cell types.
• It plays a key role in cell proliferation and migration, stimulating endothelial cell motility during microvascular morphogenesis. NG2 may also inhibit neurite outgrowth and growth cone collapse during axon regeneration.
• The extracellular domain serves as a cell surface receptor for collagen alpha-2(VI) and binds growth factors and extracellular matrix proteases, modulating their activity and contributing to tissue remodeling.
• Through its cytoplasmic C-terminus, NG2 functions as a signal transducer, recruiting scaffolding and signaling proteins to promote retraction fiber formation, cell polarization via Rho GTPase activation, and integrin-mediated adhesion through CDC42, ACK1, and BCAR1 cascades.
• In melanoma, NG2 may regulate MPP16-dependent degradation and invasion of type I collagen, enhancing tumor cell invasiveness. It also modulates the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin.
• Subcellular localization includes the cell membrane, apical cell membrane, lamellipodium membrane, cell surface, and cell projections, consistent with roles in migration and adhesion.
• Tissue-specific expression has been detected in fibroblasts, placenta, and malignant melanoma cells, with protein-level evidence supporting its relevance in both normal and cancerous contexts.
• Post-translational modifications include glycosylation and phosphorylation. The protein features disulfide bonds and repeated structural motifs, and it is classified under angiogenesis, developmental protein, differentiation, and tissue remodeling keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1583–2224 within the extracellular domain of human NG2, making it suitable for detecting cell surface expression by flow cytometry.
• Given the protein's large size (251 kDa) and extensive glycosylation, consider using optimized blocking buffers and extended incubation times to minimize non-specific binding.
• As an ABflo® 488 conjugate, this antibody is ready-to-use for flow cytometry. Validate performance in your specific experimental system, such as melanoma cell lines, and include proper isotype and fluorescence-minus-one controls.
• Reported cross-reactivity is with human NG2. If using in other species, independently verify reactivity and specificity before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including targets like CSPG4/NG2 that are pivotal for oncology and cell migration research. Our offerings range from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, all backed by technical consulting. For rare or specialized targets, our global sourcing capabilities ensure a seamless procurement experience from concept to clinic.