Rabbit anti-Human Nectin-4 Monoclonal Antibody for FC - Q96NY8

Core Product Specifications and Parameters

Biological Background: Nectin-4 Function and Localization

• Nectin-4, also known as PVRL4, LNIR, or PRR4, is a member of the nectin family of immunoglobulin superfamily cell adhesion molecules, encoded by the NECTIN4 gene in humans.
• It is a single-pass type I transmembrane protein composed of an extracellular domain with three Ig-like repeats, a transmembrane helix, and a cytoplasmic tail; multiple isoforms may exist due to alternative splicing.
• Nectin-4 mediates cell adhesion through trans-homophilic and trans-heterophilic interactions, particularly with NECTIN1, contributing to adherens junction formation and tissue organization.
• The protein is predominantly expressed in the placenta under normal physiological conditions, but is not detected in normal breast epithelium; it is, however, upregulated in breast carcinoma and other malignancies.
• Subcellularly, Nectin-4 localizes to the cell membrane and cell-cell adherens junctions; a soluble secreted form has also been reported.
• Key post-translational modifications include disulfide bond formation and glycosylation; it has been implicated in ectodermal dysplasia syndromes.
• In the context of microbial infection, Nectin-4 serves as the primary cellular receptor for measles virus entry, highlighting its role in host-pathogen interactions.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal extracellular region (amino acids 32-146) of human Nectin-4; this domain is involved in ligand recognition and virus binding, making the antibody suitable for detecting surface-expressed receptor.
• This antibody is validated for flow cytometry (FC) applications; for optimal staining, titrate the antibody and include appropriate isotype control and Fc blocking steps.
• Given the tissue specificity, consider testing on placental tissue or breast carcinoma cell lines as positive controls, and normal breast epithelial cells as a negative reference.
• Cross-reactivity has been confirmed for human; performance in other species should be verified experimentally before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For Nectin-4 research in oncology and infectious disease, our portfolio supports assay development with reliable raw material supply—from early-stage concept to clinical application. Contact us to discuss your specific sourcing needs for this target or other rare biomarkers.