Rabbit anti-Human Nectin-1/CD111 Monoclonal Antibody for FC - Q15223

Core Product Specifications and Parameters

Biological Background: Nectin-1/CD111 Function and Localization

• Nectin-1 (also known as PVRL1, CD111, HveC) is a cell adhesion molecule of the nectin family that mediates calcium-independent cell-cell adhesion through homophilic and heterophilic trans-interactions.
• It functions in nervous system development, including axon guidance, synaptogenesis, and neurite outgrowth (PubMed:21325282).
  Related references: PMID:21325282
• Heterophilic binding partners include NECTIN3 and NECTIN4, which contribute to diverse developmental processes.
• In the auditory epithelium, NECTIN1 on hair cells interacts heterophilically with NECTIN3 on supporting cells to establish a checkerboard-like cell pattern.
• Nectin-1 is required for enamel mineralization.
• Subcellularly, it localizes to the cell membrane, adherens junctions, and presynaptic membrane, with a secreted isoform also produced.
• It serves as a major host cell receptor for herpes simplex virus 1 (HSV-1) and also mediates entry of HSV-2 and pseudorabies virus (PubMed:21980294, PubMed:25231300, PubMed:28381567, PubMed:34587223).
  Related references: PMID:21980294, PMID:25231300, PMID:28381567, PMID:34587223
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination, which may regulate its stability and interactions.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , a bright far-red fluorophore optimized for flow cytometry; ensure compatible laser (e.g., 633–647 nm) and detector settings.
• For FC, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Nectin-1 is expressed on the cell surface; gentle live-cell staining protocols are recommended to preserve epitope integrity.
• Validate staining in Nectin-1-positive human cell lines (e.g., epithelial, neuronal, or keratinocyte lines) and include appropriate isotype controls.
• Avoid harsh fixation methods that may alter the recombinant immunogen's epitope conformation (amino acids 1–334).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, providing a comprehensive portfolio of validated antibodies, bulk ancillary reagents, and customized sourcing for rare targets. The anti-Nectin-1 antibody supports research in cell adhesion, neurodevelopment, and viral entry mechanisms—areas critical for assay development. We assist diagnostic manufacturers and laboratories in securing high-quality monoclonal antibodies, recombinant proteins, and technical support to accelerate your project from concept to clinic.