Rabbit anti-Human Nectin-1/CD111 mAb for FC - Q15223

Core Product Specifications and Parameters

Biological Background: Nectin-1 Function and Localization

• Nectin-1 (CD111) is a cell adhesion molecule belonging to the nectin family, which mediates homophilic and heterophilic interactions essential for cell-cell junctions and nervous system development.
  Related references:
  PMID:21325282
• It functions as a key organizer of adherens junctions and promotes axon guidance, synaptogenesis, and neurite outgrowth, playing a critical role in the formation of neural circuits.
• Nectin-1 heterophilically interacts with NECTIN3 and NECTIN4, facilitating specialized cell adhesion such as between hair cells and supporting cells in the auditory epithelium, and is required for enamel mineralization.
• As a viral entry receptor, Nectin-1 serves as the primary mediator for herpes simplex virus-1 (HSV-1) and HSV-2 entry, and also permits pseudorabies virus infection.
  Related references:
  PMID:21980294, PMID:25231300, PMID:28381567, PMID:34587223
• The protein is localized to the cell membrane, including adherens junctions and presynaptic membranes, and a secreted isoform also exists.
• Post-translational modifications include phosphorylation, ubiquitination, and disulfide bond formation, which regulate its stability and function.
• Alternative splicing produces isoforms with predicted molecular weights of 57 kDa (canonical), 51 kDa, and 39 kDa, as noted by the supplier.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 1-334) of human Nectin-1, suggesting that the antibody recognizes an epitope exposed on the cell surface, making it suitable for flow cytometry.
• For flow cytometry, use cells that express Nectin-1 on the plasma membrane; fixation and permeabilization may alter epitope accessibility, so optimization is advised.
• Titrate the conjugate to achieve optimal staining index; include appropriate isotype controls and viability dyes to ensure specific detection.
• The fluorophore is compatible with standard 594 nm laser lines; avoid exposure to light to prevent photobleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop supplier of IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for diagnostic development. This anti-Nectin-1/CD111 monoclonal antibody supports research in cell adhesion, neurodevelopment, and viral entry, and is part of CamelBio's comprehensive portfolio for infectious disease and neuroscience applications.