Rabbit anti-Human NAT10 Monoclonal Antibody for Intracellular Flow Cytometry - Q9H0A0

Core Product Specifications and Parameters

Biological Background: NAT10 Function and Localization

• NAT10 is an RNA cytidine acetyltransferase that catalyzes N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA, and tRNAs, identified as N-acetyltransferase 10 (hALP). Related references: PMID:25411247 PMID:25653167 PMID:30449621
• mRNA ac4C modification is broadly distributed, frequently at wobble cytidine sites, and enhances mRNA stability and translation efficiency. Related references: PMID:30449621 PMID:35679869
• Mediates ac4C formation at position 1842 of 18S rRNA and is required for early nucleolar cleavages during 18S rRNA synthesis. Related references: PMID:25411247 PMID:25653167
• Possesses in vitro protein lysine acetyltransferase activity toward histones, microtubules, p53, and MDM2, though the in vivo relevance remains uncertain. Related references: PMID:14592445 PMID:17631499 PMID:19303003 PMID:30449621
• Activates telomerase by stimulating TERT transcription and may affect telomerase subunit assembly and localization. Related references: PMID:14592445 PMID:18082603
• Involved in centrosome duplication control by acetylating CENATAC during mitosis, promoting SASS6 degradation. Related references: PMID:31722219
• As part of the SSU processome, participates in early ribosome biogenesis and pre-rRNA processing. Related references: PMID:34516797
• Localized to the nucleolus, nucleus, and midbody, consistent with its roles in RNA modification, ribosome assembly, and cell division.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 750–1025 of human NAT10; thus, the antibody may recognize full-length NAT10 and isoforms containing this C‑terminal region.
• Designed for intracellular flow cytometry; ensure adequate cell permeabilization before staining.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental setup.
• Validate with human cell lines known to express NAT10 (e.g., HeLa or HEK293) as a positive control, and include an appropriate isotype control.
• For multiplex analysis, confirm that the conjugate is compatible with other fluorophores used in the panel.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high‑quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti‑NAT10 conjugate. Whether you need bulk ancillary reagents, rare‑target raw‑material sourcing, or technical support for nucleolar biology and RNA modification research, our one‑stop procurement solutions help streamline your development from concept to clinic.