Rabbit anti-Human MRC2 Monoclonal Antibody for Flow Cytometry - Q9UBG0

Core Product Specifications and Parameters

Biological Background: MRC2 Function and Localization

• MRC2 (C-type mannose receptor 2), also known as ENDO180, CD280, UPARAP, or CLEC13E, is a transmembrane glycoprotein belonging to the C-type lectin receptor family.
• It functions as a calcium-dependent endocytic receptor, internalizing glycosylated ligands via clathrin-mediated endocytosis and releasing them in endosomal compartments.
• MRC2 plays a key role in collagen turnover: it binds and mediates cellular uptake of extracellular collagen for lysosomal degradation, contributing to extracellular matrix (ECM) remodeling.
• The receptor modulates plasminogen activation by regulating cell surface levels of urokinase-type plasminogen activator receptor (PLAUR) and its ligand PLAU, thereby controlling pericellular proteolysis.
• MRC2 is implicated in cancer progression and chronic tissue-destructive diseases through its effects on collagen matrix turnover, often cooperating with matrix metalloproteinases (MMPs).
• It is ubiquitously expressed at low levels in most tissues, with higher expression in fetal lung and kidney, and is present on endothelial cells, fibroblasts, and macrophages.
• According to UniProt, MRC2 is a single-pass membrane protein with a signal peptide, multiple C-type lectin domains, fibronectin type-II domain, and a short cytoplasmic tail; it is subject to disulfide bond formation, N-linked glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• This antibody is conjugated to and optimized for flow cytometry; consider titrating the antibody and validating in your cell system for optimal staining.
• The immunogen corresponds to the extracellular domain (aa 35-513), suggesting recognition of the MRC2 ectodomain; confirm accessibility in live-cell staining assays.
• Use positive control cells known to express MRC2, such as fibroblasts, macrophages (especially M2-polarized), or cancer cell lines; collagen stimulation may upregulate surface MRC2.
• For intracellular staining, validate permeabilization conditions as MRC2 cycles between the membrane and endosomal compartments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-MRC2 antibody alongside validated antibody pairs, recombinant proteins, and bulk ancillary reagents. Our integrated supply network supports MRC2-related ECM and cancer research with custom monoclonal and polyclonal antibody production, as well as rare target sourcing. Contact us to streamline your workflow from early-stage development to clinical application.