Rabbit anti-Human MRC2 mAb - Q9UBG0

Core Product Specifications and Parameters

Biological Background: MRC2 Function and Localization

• MRC2 (C-type mannose receptor 2) is also known as CD280, UPARAP, CLEC13E, or ENDO180, and belongs to the C-type lectin domain family.
• It functions as an endocytic lectin receptor with calcium-dependent lectin activity, internalizing glycosylated ligands through clathrin-mediated endocytosis.
• MRC2 is involved in the plasminogen activation system by regulating the extracellular levels of PLAUR (urokinase receptor) and PLAU (urokinase), thereby controlling cell surface protease activity.
• It contributes to the uptake, remodeling, and degradation of extracellular collagen matrices, and may collaborate with matrix metalloproteinases (MMPs).
• MRC2 plays a role in cancer progression and chronic tissue-destructive diseases through its effects on collagen turnover.
• The protein is a single-pass type I membrane protein localized at the cell membrane, with a molecular weight of approximately 167 kDa (precursor ~166.7 kDa).
• It is ubiquitously expressed at low levels in adult tissues such as brain, placenta, lung, kidney, pancreas, spleen, thymus, and colon, and highly expressed in fetal lung and kidney; it is found in endothelial cells, fibroblasts, and macrophages.
• Post-translational features include N-glycosylation, disulfide bonds, phosphorylation, calcium-binding, and a signal peptide; it is subject to ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 35-513) of human MRC2, making this antibody suitable for detecting surface-expressed receptor by flow cytometry.
• As a rabbit monoclonal antibody, it typically offers high specificity and lot-to-lot consistency; consider re-validating in your specific experimental system.
• For flow cytometry, optimize the antibody dilution using relevant positive (e.g., MRC2-expressing cell lines) and negative controls.
• If using for other applications such as immunofluorescence or immunohistochemistry, validate the antibody's performance as it has been tested only for flow cytometry.
• Note that the conjugate provides a bright, photostable green fluorophore; ensure proper instrument settings for FITC/GFP channels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal antibodies, and ancillary reagents tailored to diagnostic development. This MRC2 antibody is suited for research into endocytosis, collagen remodeling, and cancer biology. Our extensive supply network supports procurement of rare-target reagents and bulk orders to streamline your projects from concept to clinic.