Rabbit anti-Human MICA/MICB Monoclonal Antibody for Flow Cytometry - Q29983 / Q29980

Core Product Specifications and Parameters

Biological Background: MICA Function and Localization

• MICA functions as a stress-induced ligand for the activating receptor NKG2D, which is expressed on essentially all human natural killer (NK) cells, γδ T cells, and CD8+ αβ T cells. Related references: PMID:11491531, PMID:11777960
• Engagement of NKG2D by MICA triggers NK cell-mediated cytotoxicity and cytokine production, playing a critical role in anti-tumor and anti-viral immune responses. Related references: PMID:10426993
• MICA expression is upregulated under conditions of cellular stress, such as viral or bacterial infections and DNA damage, serving as a danger signal to eliminate compromised cells.
• Subcellularly, MICA is predominantly localized to the cell membrane, with some cytoplasmic presence.
• Widely expressed in tissues except the central nervous system; particularly abundant in gastric epithelium, keratinocytes, endothelial cells, and various epithelial tumors.
• Post-translational modifications include glycosylation, disulfide bond formation, and palmitoylation; it is also a target for ubiquitination (as inferred from UniProt keywords).

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a bright and photostable fluorophore optimized for flow cytometry.
• For optimal staining, titrate the antibody using relevant human cell lines or primary cells known to express MICA/MICB under normal or stress conditions.
• Include appropriate isotype controls and consider Fc receptor blocking to reduce non-specific binding.
• Validate antibody performance in human PBMCs, tumor cell lines, or stressed epithelial cells to confirm detection of surface MICA/MICB.
• If using for intracellular staining, verify that the fixation/permeabilization protocol does not affect the epitope or fluorophore signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research groups with a comprehensive portfolio of validated antibodies, including this anti-MICA/MICB conjugate for flow cytometry. As a one-stop partner, we offer optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your immune-oncology and cellular stress research. Contact us to discuss custom sourcing solutions tailored to your diagnostic development needs.