Rabbit anti-Human MICA/MICB mAb - Q29983 / Q29980

Core Product Specifications and Parameters

Biological Background: MICA/MICB Function and Localization

• MICA is a highly glycosylated, transmembrane MHC class I-related molecule that functions as a ligand for the activating immunoreceptor KLRK1/NKG2D, expressed on NK cells, γδ T cells, and CD8αβ T cells. Related references: PMID:11491531 PMID:11777960
• Under cellular stress (e.g., viral/bacterial infection, DNA damage), MICA expression is upregulated, serving as a stress signal that triggers NK cell-mediated cytotoxicity and cytokine production upon NKG2D engagement. Related references: PMID:10426993
• MICA localizes primarily to the cell membrane, with a fraction detected in the cytoplasm, and contains a single transmembrane domain.
• Tissue expression is broad, excluding the central nervous system; it is abundant in gastric epithelium, keratinocytes, endothelial cells, and many epithelial tumors (lung, breast, kidney, ovary, prostate, colon).
• The protein undergoes alternative splicing, yielding multiple isoforms, and features post-translational modifications including N-glycosylation, disulfide bond formation, and palmitoylation (S-palmitoylation).
• MICA is categorized under keywords such as adaptive immunity, cytolysis, host-virus interaction, and Ubl conjugation, reflecting its roles in immune surveillance and pathogen response.

Experimental Guidance and Technical Tips

• The immunogen is based on the extracellular domain of MICB (aa 23-298); due to sequence homology, the antibody may recognize both MICA and MICB. Verify specificity using recombinant proteins or knockout cell lines.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration and include an appropriate isotype control.
• MICA/MICB expression is induced by cellular stress; consider pre-treating cells with stimuli (e.g., DNA damaging agents) to upregulate target expression.
• Shedding of soluble MICA/MICB can occur; use live cell gating and minimize prolonged incubations to reduce background from shed protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. This -conjugated antibody targeting MICA/MICB supports immune surveillance research and tumor immunology applications. For bulk or custom requests, our one-stop sourcing platform can also supply ancillary reagents and rare-target raw materials to accelerate your diagnostic development.