Rabbit anti-Human MERTK Monoclonal Antibody for FC - Q12866

Core Product Specifications and Parameters

Biological Background: MERTK Function and Localization

• MERTK (MerTK) is a receptor tyrosine kinase also known as Proto-oncogene c-Mer, belonging to the TAM family (Tyro3, Axl, Mer).
• It transduces signals from the extracellular matrix by binding ligands like GAS6, LGALS3, TUB, and TULP1, regulating processes such as cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis) (PubMed:32640697). Related references: PMID:32640697
• Ligand binding induces autophosphorylation, creating docking sites for downstream molecules like GRB2 and PLCG2, and activating pathways involving MAPK1/2, FAK, and RAC1, which are critical for cytoskeleton reorganization and engulfment.
• MERTK plays a key role in macrophage-mediated clearance of apoptotic cells and platelet aggregation.
• In the retinal pigment epithelium (RPE), it regulates phagocytosis of rod outer segment fragments, and mutations are linked to retinitis pigmentosa.
• It inhibits Toll-like receptor (TLR)-mediated innate immune responses by activating STAT1, which induces suppressors of cytokine signaling SOCS1 and SOCS3.
• Subcellularly, MERTK is located at the cell membrane, consistent with its function as a transmembrane receptor.
• Tissue expression: not in normal lymphocytes but in many neoplastic lines; highly expressed in testis, ovary, prostate, lung, and kidney, with lower levels in spleen, small intestine, colon, and liver.
• Keywords: ATP-binding, kinase, transmembrane, glycoprotein, disulfide bond, signaling, proto-oncogene, and disease-associated (retinitis pigmentosa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 26-499) of human MERTK, suggesting the antibody may recognize an epitope in this domain; optimal performance may depend on target conformation.
• For flow cytometry, use live or appropriately fixed cells; since MERTK is a cell surface receptor, surface staining might not require permeabilization, but validate in your system.
• Include appropriate isotype controls and titrate the antibody to minimize nonspecific binding.
• Consider using known MERTK-expressing cell lines (e.g., certain cancer cell lines) as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. For MERTK-related research in oncology, immunology, or ophthalmology, we offer one-stop sourcing for monoclonal/polyclonal antibodies and custom services from concept to clinic. Contact us to explore how we can support your diagnostic development needs.