Rabbit anti-Human MERTK Monoclonal Antibody for FC - Q12866

Core Product Specifications and Parameters

Biological Background: MERTK Function and Localization

• MERTK (Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK) is a receptor tyrosine kinase with extracellular immunoglobulin and fibronectin type III domains, a single transmembrane helix, and an intracellular kinase domain.
• MERTK binds ligands such as LGALS3, TUB, TULP1, and GAS6, leading to receptor dimerization and autophosphorylation of intracellular tyrosine residues, which create docking sites for downstream signaling proteins.
• Upon activation, MERTK recruits GRB2 or PLCG2 and triggers phosphorylation of MAPK1/2, FAK/PTK2, and RAC1, mediating cell survival, migration, differentiation, and phagocytosis of apoptotic cells. Related references: PMID:32640697
• In the retinal pigment epithelium (RPE), MERTK is essential for the daily phagocytosis of shed rod outer segments, a process critical for retinal health; mutations are linked to retinitis pigmentosa.
• MERTK negatively regulates Toll-like receptor (TLR)-mediated innate immune responses by activating STAT1, which selectively induces SOCS1 and SOCS3 expression.
• Subcellularly, MERTK resides primarily at the cell membrane, consistent with its role as a transmembrane receptor.
• MERTK is not expressed in normal B- and T-lymphocytes but is highly expressed in testis, ovary, prostate, lung, kidney, and various neoplastic B- and T-cell lines, with lower levels in spleen, small intestine, colon, and liver.
• Post-translational modifications include N-glycosylation and disulfide bond formation, and the protein is phosphorylated upon activation.

Experimental Guidance and Technical Tips

• As the immunogen covers the extracellular domain (amino acids 26–499), this antibody is expected to recognize native surface MERTK; consider validating its specificity in relevant human cell lines known to express MERTK (e.g., testis, ovarian, prostate, or neoplastic B/T-cell lines). For flow cytometry, a standard direct staining protocol is recommended; titrate the antibody to determine the optimal concentration for your specific cell system.
• Include proper isotype controls and use freshly harvested cells to minimize non-specific binding. MERTK expression can be upregulated under certain conditions; verify expression levels in your experimental model beforehand.
• Shipping condition is ice bag; upon receipt, aliquot and store at recommended temperatures (typically -20°C or -80°C) and avoid repeated freeze-thaw cycles to preserve antibody activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting researchers and diagnostic developers with validated monoclonal antibodies like this anti-MERTK conjugate, optimized for flow cytometry applications. Whether you are developing assays for cancer biomarker profiling, investigating efferocytosis, or studying immune checkpoint pathways, we offer bulk antibody supply, complementary antibody pairs, and ancillary reagents to accelerate your MERTK-related immunoassay development. Our one-stop platform streamlines procurement—from initial candidate screening to scaled-up manufacturing—empowering your project from concept to clinic.