Rabbit anti-Human MERTK mAb for FC - Q12866

Core Product Specifications and Parameters

Biological Background: MERTK Function and Localization

• MERTK, also known as Tyrosine-protein kinase Mer or c-Mer, is a receptor tyrosine kinase that binds ligands such as LGALS3, TUB, TULP1, and GAS6 to transduce extracellular matrix signals.
• Upon ligand binding, MERTK autophosphorylates and provides docking sites for downstream adaptors like GRB2 and PLCG2, leading to activation of MAPK1/2, FAK/PTK2, and RAC1, thereby regulating cell survival, migration, and differentiation.
• A critical function of MERTK is the phagocytosis of apoptotic cells (efferocytosis) by macrophages, which involves cytoskeleton reorganization and engulfment (PubMed:32640697). Related references: PMID:32640697
• In the retinal pigment epithelium (RPE), MERTK is essential for the phagocytosis of rod outer segment fragments, crucial for photoreceptor renewal.
• MERTK negatively regulates Toll-like receptor (TLR)-mediated innate immune responses by activating STAT1, which induces suppressors of cytokine signaling SOCS1 and SOCS3.
• Subcellularly, MERTK is localized to the cell membrane with a single transmembrane helix; it contains immunoglobulin domains, a kinase domain, and is modified by disulfide bonds and glycosylation.
• Tissue expression is highest in testis, ovary, prostate, lung, and kidney, with lower levels in spleen, small intestine, colon, and liver; it is not expressed in normal lymphocytes but is present in neoplastic B- and T-cell lines.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fragment covering the extracellular domain (amino acids 26-499) of human MERTK, which includes the ligand-binding region. This design favors recognition of native MERTK on the cell surface, making it suitable for flow cytometry applications.
• Since the antibody is directly conjugated to , no secondary detection reagent is required. Users should titrate the antibody to determine the optimal concentration for their specific cell type and experimental conditions.
• For flow cytometry, consider including Fc receptor blocking steps and appropriate isotype controls to minimize non-specific binding.
• MERTK is a transmembrane protein; ensure that cell preparation methods (e.g., trypsinization) do not compromise surface epitope integrity. Validation with known positive and negative cell lines (e.g., MERTK-expressing cancer lines vs. lymphocytes) is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, clinical laboratories, and research institutes a comprehensive platform for sourcing premium IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target antibody development. For your MERTK-related projects—whether in oncology, immunology, or ophthalmology research—CamelBio streamlines procurement with reliable, one-stop access to critical reagents, backed by technical consultation from concept to clinic.