Rabbit anti-Human Megalin/LRP2 Monoclonal Antibody for IF-P and FC (intra) - P98164

Core Product Specifications and Parameters

Biological Background: Megalin/LRP2 Function and Localization

• Megalin (LRP2) is a 521 kDa type I transmembrane glycoprotein belonging to the low-density lipoprotein receptor family, containing multiple ligand-binding repeats and EGF-like domains.
• Functions as a multiligand endocytic receptor, primarily at the apical surface of epithelial cells, mediating the uptake of lipoproteins, vitamins, hormones, and drugs in cooperation with cubilin.
• Mediates endocytosis and transport of leptin across the blood-brain barrier and within neuronal cells, contributing to hypothalamic leptin signaling and body weight regulation (By similarity).
• In the kidney, mediates renal reabsorption of 25-hydroxyvitamin D3 bound to GC/DBP, and clears metallothionein-bound heavy metals. Related references: PMID:15126248
• Plays a crucial role in kidney homeostasis by internalizing selenoprotein SEPP1 for selenium uptake and the antiapoptotic protein BIRC5/survivin for functional integrity. Related references: PMID:23825075
• Subcellular localization includes the apical cell membrane, endosome lumen, and coated pits; also present in neuronal dendrites and axons, indicating roles in polarized trafficking.
• Essential for embryonic development, including neural tube closure via folate receptor-mediated uptake and regulation of sonic hedgehog signaling.
• Expressed predominantly in absorptive epithelia such as renal proximal tubules and placental cytotrophoblasts, with implications for nutrient and steroid hormone transport.

Experimental Guidance and Technical Tips

• The immunogen maps to the C-terminal cytoplasmic domain (aa 4446-4655), making the antibody suitable for detecting intracellular epitopes after permeabilization.
• For immunofluorescence (IF-P), ensure adequate permeabilization (e.g., Triton X-100) to allow antibody access to the intracellular domain.
• For intracellular flow cytometry (FC intra), optimize fixation and permeabilization steps to maintain antigenicity; consider using a nuclear envelope marker for gating.
• The antibody cross-reacts with mouse Megalin, enabling studies in mouse models; however, perform initial validation in your specific tissue or cell type.
• Due to the protein’s large size (521 kDa), confirm specificity using knockdown (siRNA), knockout, or blocking peptide controls.
• Pair with anti-cubilin antibodies for co-localization studies, as both receptors are involved in endocytic processes in the proximal tubule.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies on endocytic receptors and renal pathophysiology, we offer a wide range of antibodies targeting LRP2/Megalin and related proteins, along with custom sourcing for rare targets. Our integrated supply chain and technical support help streamline your diagnostic development from concept to clinic.