Rabbit anti-Human Megalin/LRP2 Monoclonal Antibody for Flow Cytometry (Intracellular) - P98164

Core Product Specifications and Parameters

Biological Background: Megalin/LRP2 Function and Localization

• Megalin, also known as LRP2 or gp330, is a large (521 kDa) transmembrane receptor belonging to the low-density lipoprotein receptor family, encoded by the LRP2 gene in humans.
• It functions as a multiligand endocytic receptor, partnering with cubilin to mediate clearance of a wide range of ligands including lipoproteins, vitamins, hormones, and drugs from the glomerular filtrate and other extracellular fluids.
• In the kidney proximal tubule, megalin mediates reabsorption of essential molecules such as 25-hydroxyvitamin D3, selenoprotein P, myoglobin, and matrix metalloproteinase 2 complexes, contributing to renal homeostasis.
• It plays a critical role in the renal handling of heavy metals by internalizing metallothionein-bound cadmium and other toxic metals, a process supported by experimental evidence. Related references: PMID:15126248
• Megalin also internalizes the anti-apoptotic protein survivin/BIRC5, which may protect kidney cells from damage during ischemia or injury. Related references: PMID:23825075
• Beyond the kidney, megalin is expressed in absorptive epithelia, including the placenta and choroid plexus, where it mediates leptin transport across the blood-brain barrier and facilitates hormone-bound vitamin uptake.
• Subcellularly, megalin localizes to the apical cell membrane, clathrin-coated pits, and endosomes, and is also detected in neuronal cell projections such as dendrites and axons, underscoring its role in neuroepithelial signaling.
• Keywords associated with megalin include endocytosis, glycoprotein, deafness, neurogenesis, and disease variant, reflecting its broad physiological importance and link to human pathologies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular region of human megalin (amino acids 4446–4655), making this antibody suitable for intracellular flow cytometry after cell permeabilization.
• Optimize fixation and permeabilization protocols for your specific cell type to ensure antibody accessibility to the cytoplasmic epitope.
• Titrate the antibody to determine the optimal concentration for staining; typical starting dilutions can be determined using positive control cells such as renal proximal tubule epithelial cells or placental cytotrophoblasts.
• Validate specificity using LRP2‑knockdown or negative cell lines to confirm minimal non‑specific binding.
• Note that the conjugate is photostable and bright, but avoid prolonged light exposure and use appropriate filter sets for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting. For scientists investigating megalin/LRP2 in endocytosis, renal physiology, or developmental biology, we offer a comprehensive portfolio of validated antibodies—including this conjugate—along with optimized antibody pairs, bulk ancillary reagents, and custom sourcing solutions for rare targets. Let us streamline your assay development from concept to clinic.