Rabbit anti-Human LIFR/CD118 Monoclonal Antibody for FC - P42702

Core Product Specifications and Parameters

Biological Background: LIFR/CD118 Function and Localization

• LIFR, also known as CD118, is the receptor for leukemia inhibitory factor (LIF) and a shared signal-transducing subunit for several cytokines.
• It forms membrane complexes either with IL6ST/gp130 alone or within the ciliary neurotrophic factor receptor (CNTFR) complex comprising CNTFR, IL6ST/gp130, and LIFR. Related references: PMID:11285233 PMID:11294841 PMID:1542794
• Ligand binding induces heterodimerization of IL6ST/gp130 and LIFR, leading to activation of JAK tyrosine kinases and subsequent phosphorylation of STAT transcription factors. Related references: PMID:11285233 PMID:11294841 PMID:9030543
• LIFR engages site 3 of cytokine ligands, a critical interaction for receptor activation. Related references: PMID:36930708
• It may also negatively regulate LIF signaling by acting as a soluble decoy receptor, blocking LIF binding to target cells.
• Subcellular localization includes the cell membrane and a secreted isoform, consistent with its dual role as a transmembrane receptor and potential shed mediator.
• The receptor is a glycoprotein with disulfide bonds and is subject to alternative splicing; chromosomal rearrangements involving LIFR are associated with certain disease variants.
• Its predicted molecular weight is approximately 124 kDa, in agreement with the product specification (UniProt: 123,743 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 45–833 of human LIFR, covering the entire extracellular domain. This design may enable recognition of both membrane-bound and shed forms of LIFR.
• This antibody is directly conjugated to ABflo 647, eliminating the need for a secondary antibody in flow cytometry; optimize the working concentration by titration in your system.
• When setting up FC, include appropriate isotype controls and use human cell lines or primary cells known to express LIFR (e.g., certain leukocytes or stem cells) as positive references.
• Because LIFR can be released from the cell surface, avoid prolonged washing steps that might remove the shed protein if the goal is to assess membrane expression.
• Given the monoclonal nature, minimal lot-to-lot variability is expected, but validation in the relevant sample matrix is recommended before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to accelerating diagnostic and research workflows by providing a comprehensive portfolio of IVD raw materials. For projects targeting LIFR signaling and flow cytometry applications, we supply validated antibodies (including this ABflo 647 conjugate), matched antibody pairs, recombinant proteins, and auxiliary reagents in bulk quantities. Whether you need standardized products or support sourcing rare-target raw materials, CamelBio bridges the gap from concept to clinical development.