Rabbit anti-Human LAMP2 Monoclonal Antibody for IF/ICC and Intracellular FC - P13473

Core Product Specifications and Parameters

Biological Background: LAMP2 Function and Localization

• LAMP2 (Lysosome-associated membrane glycoprotein 2), also known as CD107b, LAMP-2, LGP-96, or LGP110, is a heavily glycosylated transmembrane protein encoded by the LAMP2 gene. Through alternative splicing, it produces three major isoforms: LAMP-2A, LAMP-2B, and LAMP-2C, which differ in their cytosolic tails and exhibit distinct tissue distributions.
• As a key lysosomal membrane glycoprotein, LAMP2 plays an essential role in lysosome biogenesis and pH regulation by acting as a direct inhibitor of the proton channel TMEM175, thereby facilitating lysosomal acidification and optimal hydrolase activity. Related references: PMID:37390818
• LAMP2 is a central mediator of chaperone-mediated autophagy (CMA), a selective degradation pathway. It binds cytosolic chaperone complexes (e.g., HSPA8/HSC70) that deliver target proteins such as GAPDH, GPX4, NLRP3, and MLLT11 to the lysosomal membrane, where LAMP2 acts as a receptor to translocate substrates into the lysosome for degradation. Related references: PMID:11082038, PMID:18644871, PMID:24880125, PMID:36586411
• LAMP2 is required for the fusion of autophagosomes with lysosomes during macroautophagy; cells lacking LAMP2 fail to accumulate STX17 on autophagosomes, which prevents fusion and impairs degradation of autophagic cargo. Related references: PMID:27628032
• LAMP2 facilitates efficient MHC class II-mediated presentation of exogenous antigens by enabling lysosomal degradation and subsequent loading of antigenic peptides onto nascent MHC II molecules, though it is dispensable for endogenous antigen presentation. Related references: PMID:15894275, PMID:20518820
• Subcellularly, LAMP2 localizes primarily to the lysosomal membrane, but is also found in endosomal, autophagosomal, and plasma membranes, reflecting its dynamic trafficking and functions in endolysosomal and autophagic pathways.
• Isoform-specific expression patterns are observed: LAMP-2A is highly expressed in placenta, lung, and liver, while LAMP-2B and LAMP-2C show distinct distributions across tissues such as spleen, thymus, brain, and small intestine. Related references: PMID:26856698, PMID:7488019
• Notably, LAMP2 is associated with Danon disease, a glycogen storage disorder, and its study involves keywords such as alternative splicing, autophagy, disulfide bond, glycoprotein, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29-375 of human LAMP2, covering a large luminal domain common to all isoforms; this broad coverage supports detection of multiple LAMP2 variants where sequence homology permits.
• The antibody is directly conjugated to , enabling one-step staining for immunofluorescence (IF/ICC) and intracellular flow cytometry (FC intra) without the need for secondary antibodies.
• For intracellular flow cytometry, fixation and permeabilization are required; use standard protocols (e.g., 4% PFA fixation followed by saponin or Triton X-100 permeabilization) and optimize incubation times and antibody concentrations for your specific cell type.
• Validate antibody performance in the relevant biological system (human) and include appropriate positive and negative controls (e.g., wild-type versus LAMP2-knockdown cells) to confirm specificity.
• Since LAMP2 is heavily glycosylated and exists in multiple isoforms, apparent molecular weight by Western blot may differ from the predicted 44-45 kDa; testing by WB is not listed, so if needed, consider pilot experiments to verify detection capability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consultative support from concept to clinic. For researchers and diagnostic developers focusing on lysosomal biology, autophagy, or immune presentation pathways, we offer ready-to-use monoclonal antibodies like this anti-LAMP2 conjugate, as well as validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare-target raw materials to streamline your assay development.