Rabbit anti-Human LAMP1/CD107a Monoclonal Antibody for Intracellular Flow Cytometry - P11279

Core Product Specifications and Parameters

Biological Background: LAMP1/CD107a Function and Localization

• LAMP1/CD107a, encoded by the LAMP1 gene, is a heavily glycosylated lysosomal membrane protein also known as LGP120 and CD107a.
• It plays a central role in lysosome biogenesis and pH regulation by inhibiting the proton channel TMEM175, thereby facilitating lysosomal acidification necessary for hydrolase activity (PubMed:37390818). Related references: PMID:37390818
• In NK cells, LAMP1/CD107a is critical for cytotoxicity by mediating granule movement and perforin trafficking to lytic granules, and it protects NK cells from self-damage by their own cytolytic contents (PubMed:2022921, 23632890, 23847195). Related references: PMID:2022921 PMID:23632890 PMID:23847195
• Presents carbohydrate ligands to selectins, contributing to cell adhesion (PubMed:7685349). Related references: PMID:7685349
• Acts as a receptor for Lassa virus, promoting viral entry and fusion, and supports FURIN-mediated cleavage of mumps virus fusion protein (PubMed:24970085, 25972533, 27605678, 29295909). Related references: PMID:24970085 PMID:25972533 PMID:27605678 PMID:29295909
• Subcellularly, LAMP1/CD107a localizes to lysosomes, late endosomes, endosomes, cell membrane, and cytolytic granule membranes.
• Post-translational modifications include extensive N-linked glycosylation, disulfide bond formation, and alternative splicing.

Experimental Guidance and Technical Tips

• Since this antibody targets an intracellular epitope (immunogen: amino acids 29-382), it is intended for intracellular flow cytometry. Optimize permeabilization and fixation protocols using appropriate controls.
• is a bright far-red fluorophore; ensure proper compensation when multiplexing with other dyes and use an unlabeled control to check for non-specific binding.
• Validate antibody staining in human cell lines expressing LAMP1/CD107a; typical lysosomal staining patterns can be confirmed by co-localization with lysosomal markers.
• For degranulation assays (e.g., NK cell activation), this intracellular antibody requires permeabilization, so it may detect total cellular protein. Adjust gating strategies accordingly.
• Store at recommended conditions and protect from light to preserve fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to your project. For the LAMP1/CD107a target—central to lysosome biology, autophagy, and immuno-oncology research—we offer reliable raw materials and sourcing solutions to accelerate your assay development.