Rabbit anti-Human LAMP1/CD107a Monoclonal Antibody for Flow Cytometry (Intracellular) - P11279

Core Product Specifications and Parameters

Biological Background: LAMP1/CD107a Function and Localization

• Lysosomal membrane glycoprotein crucial for lysosome biogenesis, pH regulation, autophagy, and cholesterol homeostasis. Acts as a direct inhibitor of the proton channel TMEM175 to facilitate lysosomal acidification. Related references: PMID:37390818
• Critical mediator of NK cell cytotoxicity: participates in cytotoxic granule movement, perforin trafficking, and protects NK cells from self-degranulation damage. Related references: PMID:2022921 PMID:23632890 PMID:23847195
• Presents carbohydrate ligands to selectins, contributing to cell adhesion processes. Related references: PMID:7685349
• Functions as a receptor for Lassa virus glycoprotein and promotes viral fusion in less acidic endosomes. Related references: PMID:24970085 PMID:25972533 PMID:27605678 PMID:29295909
• Supports FURIN-mediated cleavage of mumps virus fusion protein F through interaction with FURIN and the unprocessed viral protein.
• Localizes to lysosome, late endosome, cell membrane, and cytolytic granule membranes. Undergoes alternative splicing and is highly glycosylated with disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 29–382 of human LAMP1, covering the majority of the luminal domain; consider this when designing detection assays.
• For intracellular flow cytometry, perform cell fixation and permeabilization prior to staining; optimize permeabilization protocol for your cell type.
• The conjugate is suitable for violet laser excitation; include appropriate compensation controls in multicolor panels.
• Validate antibody performance in LAMP1-expressing cell lines (e.g., HeLa, Jurkat) and relevant primary cells such as NK cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. This LAMP1/CD107a antibody supports research into lysosomal biology, cancer, and immune cell function. Leverage our one-stop sourcing capability to secure rare-target raw materials and accelerate your assay development from concept to clinic.