Rabbit anti-Human LAMP1/CD107a Monoclonal Antibody for Flow Cytometry (Intracellular) - P11279

Core Product Specifications and Parameters

Biological Background: LAMP1/CD107a Function and Localization

• LAMP1, also known as CD107a or LGP120, is a heavily glycosylated lysosomal membrane protein involved in lysosome biogenesis and homeostasis.
• It regulates lysosomal pH by directly inhibiting the TMEM175 proton channel, facilitating acidification for optimal hydrolase activity. Related references: PMID:37390818
• LAMP1 plays a critical role in NK cell cytotoxicity: it mediates cytotoxic granule movement, perforin trafficking, and protects NK cells from self-inflicted damage. Related references: PMID:2022921, PMID:23632890, PMID:23847195
• The protein presents carbohydrate ligands to selectins, supporting cell adhesion. Related references: PMID:7685349
• LAMP1 acts as a host cell receptor for Lassa virus glycoprotein and promotes virus-host membrane fusion. Related references: PMID:24970085, PMID:25972533, PMID:27605678, PMID:29295909
• It also supports FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and unprocessed viral F protein.
• Subcellularly, LAMP1 localizes to lysosome, late endosome, endosome, cell membrane, and cytolytic granule membranes.
• The mature glycoprotein migrates at approximately 38–45 kDa in SDS-PAGE due to heavy glycosylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 29-382) covers the luminal domain of LAMP1, suggesting the antibody recognizes an epitope in this region.
• For intracellular flow cytometry (FC intra), optimize fixation and permeabilization conditions to enable antibody access to lysosomal compartments.
• Include appropriate isotype controls and validate staining in relevant human cell lines or primary samples.
• LAMP1 can transiently appear on the cell surface; thus, evaluate both surface and intracellular staining protocols as needed.
• Due to glycosylation heterogeneity, the observed molecular weight in western blot may range from 38 to 45 kDa; confirm band specificity if adapting to other applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing diagnostic manufacturers and research labs with one-stop access to high-quality reagents. This anti-human LAMP1 monoclonal antibody supports studies in lysosome biology, autophagy, and immune cell cytotoxicity. In addition to individual antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development.