Rabbit anti-Human LAMP1/CD107a mAb - P11279

Core Product Specifications and Parameters

Biological Background: LAMP1/CD107a Function and Localization

• LAMP1, also designated CD107a or LAMPA, is a heavily glycosylated type I transmembrane protein and a major constituent of the lysosomal membrane.
• Plays a critical role in lysosome biogenesis, autophagy, and cholesterol homeostasis, acting as a direct inhibitor of the proton channel TMEM175 to regulate lysosomal pH (PubMed:37390818). Related references: PMID:37390818
• In NK cells, LAMP1 mediates cytotoxicity by facilitating cytotoxic granule movement and perforin trafficking, while protecting NK cells from degranulation-associated damage (PubMed:2022921, PubMed:23632890, PubMed:23847195). Related references: PMID:2022921, PMID:23632890, PMID:23847195
• Presents carbohydrate ligands to selectins, contributing to cell adhesion processes (PubMed:7685349). Related references: PMID:7685349
• Facilitates Lassa virus entry by acting as a receptor and promotes viral fusion, and supports FURIN-mediated cleavage of mumps virus fusion protein (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:29295909). Related references: PMID:24970085, PMID:25972533, PMID:27605678, PMID:29295909
• Localizes primarily to lysosome, late endosome, and cytolytic granule membranes, and can transiently appear on the cell surface.
• Undergoes extensive N-glycosylation and disulfide bond formation, which are critical for its stability and function in acidic environments.
• Exhibits multiple isoforms due to alternative splicing, with apparent molecular weights of 38–45 kDa depending on glycosylation state.

Experimental Guidance and Technical Tips

• The immunogen spans the luminal domain (amino acids 29–382), favoring recognition of native intracellular LAMP1 in permeabilized cells.
• For flow cytometry, ensure adequate cell permeabilization and fixation protocols to access intracellular LAMP1; optimize antibody titration for your specific experimental system.
• The conjugate allows direct detection, reducing the need for secondary antibodies and minimizing background.
• Validate staining in LAMP1-expressing human cell lines (e.g., HeLa, Jurkat) as positive controls.
• Consider using an unconjugated primary antibody if multiplexing or if applications other than flow cytometry are required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-LAMP1 (CD107a) antibody is ideal for research into lysosome biology, autophagy, and immune cell cytotoxicity. With deep expertise in rare target sourcing, CamelBio supports your entire diagnostic development journey from concept to clinic.