Rabbit anti-Human Lambda Light Chain Monoclonal Antibody for Flow Cytometry - P0CG04

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Lambda Constant 1 Function and Localization

• Immunoglobulin lambda constant 1 (IGLC1) is the constant region of immunoglobulin light chains of the lambda type, pairing with heavy chains to form functional antibodies secreted by plasma cells or expressed on B cell membranes.
• Immunoglobulins are glycoproteins produced by B lymphocytes that mediate both antigen recognition and effector functions in adaptive humoral immunity.
• Antigen binding to membrane-bound immunoglobulins triggers B cell clonal expansion and differentiation into antibody-secreting plasma cells, driving the humoral immune response. Related references: PMID:20176268, PMID:22158414
• The variable domains of light and heavy chains are assembled by V-(D)-J rearrangement and undergo somatic hypermutation after antigen exposure, enabling affinity maturation for specific antigens. Related references: PMID:17576170, PMID:20176268
• The lambda light chain constant region contains a characteristic immunoglobulin domain stabilized by a disulfide bond and is essential for correct antibody folding and stability.
• Primarily secreted, it can also appear as Bence-Jones protein in urine under pathological conditions such as multiple myeloma.
• Subcellular localization includes the secretory pathway and the plasma membrane when part of the B cell receptor complex.
• Keywords: Adaptive immunity, Immunoglobulin, Disulfide bond, Secreted, Cell membrane.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–106 of the human lambda light chain constant region, ensuring robust reactivity with native IGLC1 in denatured and native conformations.
• This antibody is pre-conjugated to , a far-red fluorophore compatible with common flow cytometers; titrate to determine optimal staining concentration for your cell model.
• Validate specificity using relevant human B lymphocyte cell lines or peripheral blood mononuclear cells (PBMCs); include appropriate isotype controls to assess non-specific binding.
• When designing multicolor flow cytometry panels, check spectral spillover and perform compensation using single-stained controls.
• The antibody recognizes the constant region of lambda light chains and is expected not to cross-react with kappa light chains; confirm in your experimental system if dual light-chain discrimination is required.
• For storage, aliquot and protect from light to maintain fluorophore stability; avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to premium IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this -conjugated anti-human lambda light chain clone. Whether you are developing flow cytometry reagents, B cell profiling panels, or immunodiagnostic kits for humoral immunity disorders, our catalog and custom sourcing services can connect you with rare target raw materials, bulk ancillary reagents, and flexible supply solutions. Partner with CamelBio to accelerate your project from concept to clinic.