Rabbit anti-Human Lambda Light Chain Monoclonal Antibody for Flow Cytometry - P0CG04

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Lambda Constant 1 Function and Localization

• Human Immunoglobulin lambda constant 1 (IGLC1), also known as Ig lambda chain C region MGC or Ig lambda-1 chain C region, is a constant region of immunoglobulin light chains.
• Immunoglobulins, produced by B lymphocytes, serve as membrane-bound receptors or secreted antibodies. Membrane-bound forms trigger B cell clonal expansion upon antigen binding, while secreted forms mediate the elimination of antigens (humoral immunity). Related references: PMID:20176268 PMID:22158414
• Each immunoglobulin molecule includes two identical light chains; the antigen binding site is formed by the variable domain of one heavy chain and that of one light chain. Variable domains are assembled through V-(D)-J rearrangement and undergo somatic hypermutation, enabling affinity maturation for specific antigens. Related references: PMID:17576170 PMID:20176268
• The IGLC1 gene encodes this protein, and it is one of several functional constant region genes in the lambda light chain locus.
• Subcellular localization: Secreted immunoglobulins are found in serum and other body fluids; membrane-bound immunoglobulins are anchored to the B cell surface.
• Key structural features include intrachain disulfide bonds forming immunoglobulin domains. The lambda constant region is also related to Bence-Jones proteins, which are free immunoglobulin light chains found in urine of patients with multiple myeloma.
• Predicted molecular weight is approximately 11.3 kDa (calculated from sequence).

Experimental Guidance and Technical Tips

• This -conjugated antibody is suitable for flow cytometric detection of human lambda light chain expression. is a red fluorescent dye with spectral properties similar to Alexa Fluor 594, compatible with 561 nm or 594 nm lasers.
• Consider optimizing the antibody concentration for your specific sample type and fixation/permeabilization protocol. Validating in relevant cell lines or primary B cells is recommended.
• For intracellular staining, appropriate fixation and permeabilization steps may be required. Fc receptor blocking can reduce non-specific binding.
• The immunogen spans amino acids 1-106, covering the complete constant domain; thus, the antibody recognizes both membrane-bound and secreted lambda light chains.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents. This -conjugated anti-human lambda light chain antibody supports immunological research and assay development in adaptive immunity and lymphoproliferative disorder diagnostics. Count on CamelBio for reliable sourcing solutions tailored to your project requirements.