Rabbit anti-Human KLRG1 PolymAb® for Flow Cytometry - Q96E93

Core Product Specifications and Parameters

Biological Background: KLRG1 Function and Localization

• KLRG1 (Killer cell lectin-like receptor subfamily G member 1) is a type II transmembrane glycoprotein belonging to the C-type lectin superfamily; it is also known as MAFA, CLEC15A, or MAFA-L.
• The protein functions as an inhibitory receptor on natural killer (NK) cells and T-cells, primarily CD8+ T-cells, and negatively regulates their effector functions upon ligand engagement.
• KLRG1 recognizes classical cadherins such as E-cadherin (CDH1), N-cadherin (CDH2), and R-cadherin (CDH4), and this interaction is thought to mediate "missing self" recognition, sensing the integrity of cadherin-expressing tissues.
• The receptor contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain, which mediates inhibitory signaling.
• KLRG1 is expressed specifically on NK cells and T-cells, with predominant expression on CD8+ T-cell subsets; it is often used as a marker of terminally differentiated or senescent T-cells.
• Subcellularly, KLRG1 localizes to the cell membrane via a signal-anchor transmembrane domain.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are characteristic of its extracellular C-type lectin domain. Alternative splicing has been reported.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 60-195 of human KLRG1, encompassing the extracellular domain; the recombinant fusion protein elicits antibodies specific to the native conformation in flow cytometry.
• This antibody is conjugated to , a bright far-red fluorophore ideal for multicolor flow cytometry panels; consider titrating the antibody to optimize the staining index and minimize background.
• Given the restricted expression on NK and T-cell subsets, validate antibody performance in the relevant sample system (e.g., human PBMCs) and include appropriate isotype controls and Fc receptor blocking to ensure specificity.
• Note that the predicted molecular weight is 22 kDa; however, due to glycosylation, the band may appear at a higher molecular weight in western blotting if attempted.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with comprehensive IVD raw-material solutions, from concept to clinic. For projects targeting immune checkpoint receptors like KLRG1, we offer a streamlined supply of validated antibody pairs, optimized monoclonal and recombinant antibodies, bulk ancillary reagents, and assistance in sourcing rare specificities. By bridging your development needs with reliable raw materials, CamelBio helps accelerate your assay development in immuno-oncology and immune profiling applications.