Rabbit anti-Human KLRG1 PolymAb® for FC - Q96E93

Core Product Specifications and Parameters

Biological Background: KLRG1 Function and Localization

• Official full name: Killer cell lectin-like receptor subfamily G member 1; also known as MAFA (mast cell function-associated antigen), CLEC15A, and ITIM-containing receptor MAFA-L. Encoded by the KLRG1 gene.
• Functions as an inhibitory receptor on natural killer (NK) cells and T cells upon binding to non-MHC ligands. Plays a role in missing-self recognition by binding to a conserved site on classical cadherins (E-cadherin/CDH1, N-cadherin/CDH2, R-cadherin/CDH4) on target cells, thus monitoring cadherin expression.
• Belongs to the C-type lectin domain family 15 (CLEC15A) and contains a single ITIM (immunoreceptor tyrosine-based inhibitory motif) in its cytoplasmic domain, mediating inhibitory signaling.
• Localizes to the cell membrane as a single-pass type II transmembrane glycoprotein, with a signal-anchor topology.
• Expression is restricted to NK cells and T cells, predominantly CD8+ T cells, consistent with its immune regulatory role.
• Post-translational modifications include glycosylation and disulfide bond formation, and alternative splicing generates multiple isoforms (note: the provided sequence corresponds to isoform 2, according to the immunogen).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 60-195) of human KLRG1 isoform 2 (NP_001316028.1); this region encompasses the cadherin-binding C-type lectin domain, facilitating specific detection of native conformations.
• This antibody is directly conjugated to , a bright and photostable fluorophore, enabling direct flow cytometry without a secondary antibody. It is recommended for detecting KLRG1 on the surface of live human NK cells and CD8+ T cells.
• For optimal results, titrate the antibody to determine the ideal concentration for your specific cell type and experimental conditions. Include appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• Validate in the relevant sample system (e.g., human peripheral blood mononuclear cells) by co-staining with lineage markers (CD3, CD56, CD8) to confirm expected expression patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic and research raw materials, offering a wide portfolio of validated antibodies, antibody pairs, and ancillary reagents. KLRG1 is a marker of interest in immune exhaustion and aging, and we can provide bulk quantities of this ready-to-use conjugate, along with matching antibodies for multiplex panels. Our team can also assist with rare target sourcing and customized reagent development to accelerate your projects from concept to clinic.