Rabbit anti-Human KIR2DL4/CD158d Monoclonal Antibody for FC - Q99706

Core Product Specifications and Parameters

Biological Background: KIR2DL4/CD158d Function and Localization

• KIR2DL4, also known as CD158d, is a member of the killer cell immunoglobulin-like receptor (KIR) family and exhibits multiple splice variants contributing to observed protein sizes.
• It serves as a receptor for non-classical MHC class Ib HLA-G molecules, specifically recognizing peptide-bound HLA-G-B2M complexes on target cells.
• In decidual NK cells, KIR2DL4 engagement with HLA-G triggers a senescence-associated secretory phenotype that promotes vascular remodeling and supports fetal growth during early pregnancy. Related references: PMID:16366734 PMID:23184984 PMID:29262349
• This receptor plays a role in balancing maternal-fetal tolerance and antiviral immunity by regulating effector functions of NK cells, CD8⁺ T cells, and B cells. Related references: PMID:10190900 PMID:16366734
• Upon HLA-G binding, KIR2DL4 initiates signaling from the endosomal compartment, leading to activation of PRKDC-XRCC5 and AKT1, which ultimately drives NF-κB-dependent pro-inflammatory responses. Related references: PMID:20179272
• The protein localizes to the cell membrane and early endosome membrane, consistent with its signaling role from endosomes.
• Expression is predominantly observed in decidual NK cells and type 1 innate lymphoid cells (ILC1), with a detectable subset in peripheral NK cells. Related references: PMID:29262349 PMID:19304799
• KIR2DL4 is a glycoprotein with disulfide bonds; alternative splicing and post-translational modifications yield multiple isoforms ranging from approximately 24 to 41 kDa.

Experimental Guidance and Technical Tips

• The conjugate is designed for direct flow cytometry; titrate the antibody to determine the optimal working concentration for your specific cell type.
• The immunogen corresponds to the extracellular domain (amino acids 24–242), supporting recognition of surface-expressed KIR2DL4 in live cells.
• Because KIR2DL4 is expressed on NK cell subsets, combine with appropriate lineage markers (e.g., CD56, CD3) for accurate gating.
• Multiple isoforms exist; validate specificity and performance in your experimental system, as the antibody may react with various splice variants.
• For optimal staining, use freshly isolated or properly cryopreserved cells to maintain antigen integrity and minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our KIR2DL4/CD158d monoclonal antibody supports immunology and reproductive biology research applications, and we can assist with sourcing rare-target raw materials for your specific assay development needs. Partner with CamelBio to streamline your supply chain from concept to clinic.