Rabbit anti-Human KIR2DL4/CD158d mAb for FC - Q99706

Core Product Specifications and Parameters

Biological Background: KIR2DL4 Function and Localization

• KIR2DL4 is a killer cell immunoglobulin-like receptor that functions as a receptor for non-classical MHC class Ib molecule HLA-G, recognizing peptide-bound HLA-G-B2M complexes. Related references: PMID:16366734, PMID:23184984, PMID:29262349
• In decidual NK cells, KIR2DL4 engagement triggers a senescence-associated secretory phenotype, promoting vascular remodeling and fetal growth during early pregnancy. Related references: PMID:29262349
• It plays a role in balancing maternal-fetal tolerance and antiviral immunity by modulating effector functions of NK cells, CD8+ T cells, and B cells. Related references: PMID:10190900, PMID:16366734
• Upon ligand binding, KIR2DL4 initiates signaling from the endosomal compartment, leading to activation of PRKDC-XRCC5 and AKT1, and downstream NF-kappa-B-dependent pro-inflammatory responses. Related references: PMID:20179272
• Subcellularly, KIR2DL4 localizes to the cell membrane and early endosome membrane.
• Tissue expression is restricted to decidual NK cells, innate lymphoid cell type I (ILC1), and a subset of peripheral NK cells. Related references: PMID:29262349, PMID:19304799
• As a transmembrane glycoprotein with immunoglobulin-like domains, KIR2DL4 undergoes alternative splicing, resulting in multiple isoforms with varying molecular weights (24–41 kDa).
• Key structural and functional features include disulfide bonds, glycosylation, and a transmembrane helix; it is classified under the immunoglobulin-like receptor family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24-242), suggesting the antibody likely recognizes cell surface KIR2DL4. For flow cytometry, consider staining live, non-permeabilized cells.
• This -conjugated antibody is ready-to-use for flow cytometry. Protect from light and use appropriate isotype controls.
• Validate reactivity in human NK cell samples or NK cell lines known to express KIR2DL4.
• Due to alternatively spliced isoforms, multiple protein bands may be observed in western blotting; however, this product is validated only for flow cytometry, and other applications require independent validation.
• For quantitative flow cytometry, titrate the antibody to determine the optimal concentration for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio provides one-stop access to validated antibodies, recombinant proteins, and ancillary reagents for immunology and NK cell receptor research. Whether you need custom monoclonal antibody development, bulk raw materials, or rare target sourcing like KIR2DL4/CD158d, our platform bridges the gap between discovery and diagnostic application.