Rabbit anti-Human KIR2DL2/KIR2DL3 Monoclonal Antibody for IF/ICC, FC - P43628

Core Product Specifications and Parameters

Biological Background: Killer cell immunoglobulin-like receptor 2DL3 Function and Localization

• Encodes a type I transmembrane glycoprotein belonging to the killer cell immunoglobulin-like receptor (KIR) family; member of the inhibitory receptor subfamily.
• Functions as a receptor for specific HLA-C alleles (HLA-Cw1, Cw3, and Cw7), transmitting inhibitory signals to natural killer (NK) cells.
• Contains multiple immunoglobulin-like domains and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), which upon ligand binding recruits phosphatases to suppress NK cell activation.
• Key regulator of NK cell tolerance and self-recognition, preventing cytotoxic lysis of healthy cells expressing cognate HLA class I molecules.
• Undergoes alternative splicing to generate isoforms with predicted molecular weights of 27 kDa, 37 kDa, and 38 kDa (UniProt: ~38 kDa full-length).
• Demonstrates structural features including an extracellular region with two Ig-like domains, a transmembrane helix, and a cytoplasmic tail; extracellular domain contains predicted disulfide bonds and N-glycosylation sites.
• Localized at the plasma membrane, where it interacts with peptide-loaded HLA-C molecules on target cells.
• Implicated in NK cell education and immune surveillance, with relevance in hematopoietic stem cell transplantation and viral infection control.

Experimental Guidance and Technical Tips

• The recombinant immunogen (amino acids 22–245) corresponds to the extracellular domain, suggesting the antibody may recognize native conformational epitopes; consider testing with both fixed and live-cell samples.
• Being directly conjugated to , a bright and photostable fluorophore, this antibody is ideal for multiplexed immunofluorescence and flow cytometry; titrate carefully to minimize background.
• For flow cytometry, include appropriate Fc receptor blocking steps to reduce nonspecific binding, given the antibody's recognition of an immunoglobulin superfamily member.
• Validate expression in well-characterized NK cell lines (e.g., NKL, NK92) or primary human PBMCs; stimulation with cytokines may upregulate target expression.
• For immunofluorescence, optimize fixation and permeabilization protocols, as the target is a transmembrane protein; detergents may affect epitope accessibility.
• A standard, non-conjugated version of the antibody may be available for applications requiring an indirect detection system; inquire with the supplier for customization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We specialize in bridging the gap for rare targets like KIR receptors, offering sourcing solutions for immunophenotyping and NK cell research. Whether you need single-vial validation materials or large-scale production lots, CamelBio streamlines your supply chain from concept to clinic.