Rabbit anti-Human KIR2DL2/KIR2DL3 Monoclonal Antibody for IF/ICC, FC - P43628

Core Product Specifications and Parameters

Biological Background: KIR2DL2/KIR2DL3 Function and Localization

• KIR2DL3 is a killer cell immunoglobulin-like receptor that belongs to a large family of highly homologous receptors predominantly expressed on natural killer (NK) cells.
• It functions as an inhibitory receptor that specifically recognizes HLA-C alleles (HLA-Cw1, HLA-Cw3, and HLA-Cw7), delivering inhibitory signals that prevent NK cell-mediated lysis of target cells.
• The protein is a transmembrane glycoprotein featuring extracellular immunoglobulin-like domains responsible for ligand binding, and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) domain for signal transduction.
• KIR2DL3 is located on the cell membrane where it interacts with its MHC class I ligands to modulate immune responses.
• Alternative splicing generates multiple isoforms that may exhibit different functional properties.
• Post-translational modifications include disulfide bond formation and glycosylation, contributing to protein stability and function.
• The closely related gene KIR2DL2 shares high sequence homology, and the antibody cross-reacts with both due to the conserved immunogen region.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 22-245), which is likely to be accessible for immunofluorescence (IF/ICC) and flow cytometry (FC) applications.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell system, as conjugate brightness () can influence staining index.
• In IF/ICC, consider permeabilization protocols if intracellular epitopes are to be detected, although the target is a cell surface receptor; fixation may affect antibody binding.
• Include appropriate isotype and fluorescence-minus-one controls to ensure specificity, especially given the high homology among KIR family members.
• Validate the antibody in your sample system using positive (e.g., NK cell lines) and negative (e.g., KIR-negative cells) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material supplier, connecting diagnostic manufacturers and laboratories with rigorously validated reagents. For NK cell immunophenotyping and immune-oncology research, we offer ready-to-use -conjugated KIR2DL2/KIR2DL3 monoclonal antibodies, alongside a full portfolio of quality-assured ancillary reagents and custom bulk sourcing services. Our one-stop platform streamlines procurement from early-stage development to clinical validation, ensuring consistent supply for your assay workflows.