Rabbit anti-Human KIR2DL2/KIR2DL3 Monoclonal Antibody for IF/ICC and FC - P43628

Core Product Specifications and Parameters

Biological Background: KIR2DL2/KIR2DL3 Function and Localization

• The target proteins KIR2DL2 and KIR2DL3 are members of the killer cell immunoglobulin-like receptor (KIR) family. The following functional details are derived from the characterization of KIR2DL3 (UniProt P43628).
• KIR2DL3, also known as CD158b, NKAT2, and p58 NK receptor, is an inhibitory receptor expressed on natural killer (NK) cells.
• It specifically binds to HLA-C alleles (HLA-Cw1, HLA-Cw3, and HLA-Cw7), thereby suppressing NK cell activity and preventing target cell lysis.
• The receptor is a transmembrane protein localized to the cell membrane, classified as a single-pass type I membrane glycoprotein based on its structural features.
• Structurally, it contains immunoglobulin-like domains and requires disulfide bonds for proper folding, as indicated by its protein keywords.
• Alternative splicing of the KIR2DL3 gene produces multiple isoforms, which may exhibit variations in ligand specificity or signaling capacity.
• Additionally, it undergoes post-translational modifications, including glycosylation, which is common among immune receptors and influences cell-surface expression.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (aa 22-245) of KIR2DL3, which is likely to present native epitopes suitable for recognition of both KIR2DL2 and KIR2DL3 on the cell surface.
• For flow cytometry (FC), the direct conjugate eliminates the need for secondary antibody detection, reducing background and simplifying the staining protocol. Ensure appropriate compensation when combined with other fluorophores.
• In immunofluorescence (IF/ICC), fixation and permeabilization steps should be optimized based on the target's plasma membrane localization. Non-permeabilized staining can be used to detect surface-localized receptors.
• Given the receptor's restricted expression on NK cells and some T-cell subsets, use a relevant positive control such as human peripheral blood mononuclear cells (PBMCs) or NK cell lines for initial validation.
• Antibody performance may vary with different sample preparation methods; titrate the antibody (e.g., 0.1-1 µg/mL) to determine the optimal working concentration for your specific assay.
• Cross-reactivity with human KIR2DL2 is expected based on sequence homology; however, confirm specificity in your model system using appropriate knockout or knockdown controls if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For NK cell receptor and immune profiling research, we offer a comprehensive portfolio including validated antibody pairs, fluorescent conjugates like this anti-KIR2DL2/KIR2DL3 mAb, and ancillary reagents in various scales. Our expertise also extends to sourcing rare targets and custom antibody development to accelerate your assay development.