Rabbit anti-Human JAM-A/CD321/F11R Monoclonal Antibody for Flow Cytometry - Q9Y624

Core Product Specifications and Parameters

Biological Background: JAM-A Function and Localization

• JAM-A (Junctional adhesion molecule A), also known as JAM1, CD321, and F11R, is a cell adhesion molecule of the immunoglobulin superfamily localized at tight junctions and the cell membrane.
• Plays an essential role in epithelial tight junction formation by recruiting the polarity protein PARD3.
  Related references: PMID:11489913
• Serves as a ligand for integrin alpha-L/beta-2 (LFA-1), mediating memory T-cell and neutrophil transmigration across endothelial and epithelial barriers.
  Related references: PMID:11812992
• Contributes to platelet activation and aggregation, playing a part in hemostasis and thrombosis.
  Related references: PMID:10753840
• Acts as a receptor for viral entry, including Mammalian reovirus (sigma-1 protein) and Human Rotavirus strain Wa, implicating it in host-virus interactions.
• Expressed at the protein level in endothelial cells, epithelial cells, and leukocytes, consistent with its roles in barrier function and immune cell trafficking.
• Key post-translational modifications include N-glycosylation, disulfide bond formation, and phosphorylation; contains extracellular immunoglobulin-like domains.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 28–242, covering the extracellular domain. This design targets native surface-exposed epitopes, making the antibody well-suited for non-permeabilized live-cell flow cytometry.
• For human leukocyte or endothelial cell staining, perform Fc receptor blocking and use appropriate isotype controls to minimize background.
• conjugate is optimized for flow cytometry; ensure instrument laser and filter configuration match the dye’s excitation/emission profile (Ex/Em ~590/617 nm).
• Consider validating antibody specificity with JAM‑A‑overexpressing and knockout cell lines or siRNA‑mediated knockdown in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner offering one-stop access to high-quality antibodies, antigens, and ancillary reagents. For tight junction and immunology research, we supply validated monoclonal antibodies like this anti-JAM-A conjugate and support bulk sourcing for flow cytometry assay development. Whether you need custom antibody pairs, recombinant proteins, or scalable reagent supply, CamelBio streamlines your path from concept to clinic.