Rabbit anti-Human JAM-A/CD321/F11R Monoclonal Antibody for Flow Cytometry - Q9Y624

Core Product Specifications and Parameters

Biological Background: JAM-A/CD321/F11R Function and Localization

• JAM-A, encoded by the F11R gene, is a single-pass type I membrane protein and member of the immunoglobulin superfamily (IgSF). It is also known as junctional adhesion molecule 1 (JAM-1), platelet F11 receptor, and platelet adhesion molecule 1 (PAM-1).
• Plays a critical role in epithelial tight junction assembly, recruiting the PARD3 polarity protein to early cell junctions. Related references: PMID:11489913
• Functions as a ligand for integrin αL/β2 (LFA-1), facilitating memory T-cell and neutrophil transmigration across endothelial and epithelial barriers. Related references: PMID:11812992
• Involved in platelet activation; engagement of JAM-A on platelets contributes to thrombus formation and hemostasis. Related references: PMID:10753840
• Serves as a cellular receptor for certain viruses, including Mammalian reovirus sigma-1 and Human Rotavirus strain Wa, mediating viral entry.
• Localizes to tight junctions of polarized epithelial and endothelial cells, as well as the cell membrane of circulating leukocytes and platelets.
• Expressed in endothelium, epithelium, and leukocytes at the protein level.
• Post-translational modifications include N-linked glycosylation and phosphorylation; contains a signal peptide, two Ig-like C2-type domains, and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 28-242) of human JAM-A, covering both Ig-like domains; this region is well-suited for detection of surface-expressed JAM-A in live-cell flow cytometry.
• For flow cytometry, consider optimizing antibody titration and include appropriate isotype controls to assess non-specific binding.
• JAM-A is expressed on endothelial cells, epithelial cells, and leukocytes; validate expression in your target sample using positive (e.g., activated leukocytes) and negative controls.
• The conjugate is compatible with standard flow cytometers equipped with a red laser (~647 nm); protect from light during staining and handling.
• For adherent cells, use gentle dissociation methods to preserve surface epitopes.
• Cross-reactivity is reported for human; to use with other species, verify sequence homology and perform empirical validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories integrated access to IVD raw materials, bridging the gap from concept to clinical application. For tight junction and cell adhesion research, this anti-JAM-A antibody supports flow cytometry workflows, backed by our comprehensive portfolio of validated antibody pairs, bulk ancillary reagents, and custom raw material sourcing. Partner with us to accelerate your assay development with reliable, high-performance reagents.