Rabbit anti-Human ITGA1/CD49a Monoclonal Antibody for FC - P56199

Core Product Specifications and Parameters

Biological Background: Integrin alpha-1 Function and Localization

• Integrin alpha-1 (ITGA1), also known as CD49a or VLA-1, is a type I transmembrane glycoprotein that pairs with integrin beta-1 to form a heterodimeric receptor for laminin and collagen.
• It specifically binds to the proline-hydroxylated sequence G-F-P-G-E-R in collagen, mediating cell adhesion and anchorage-dependent regulation of cell growth.
• Subcellular localization: Expressed at the cell membrane as a single-pass membrane protein.
• Involved in negative regulation of EGF-stimulated cell growth, indicating a role in growth factor signaling modulation.
• Contains multiple integrin alpha repeats and binds divalent cations (calcium and magnesium), which are required for ligand binding and structural integrity.
• Post-translational modifications include glycosylation and disulfide bond formation.
• Keywords: Cell adhesion, Calcium, Glycoprotein, Integrin, Magnesium, Membrane, Metal-binding, Receptor, Repeat, Transmembrane.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 29-1141), targeting surface-exposed epitopes ideal for non-permeabilized flow cytometry.
• For FC applications, titrate the conjugate to determine the optimal staining concentration and avoid non-specific binding. Validate in human cell lines known to express ITGA1, such as activated T lymphocytes or fibroblasts.
• Use appropriate isotype controls and consider Fc receptor blocking to ensure specific detection.
• The fluorophore offers bright and photostable signals, enabling direct one-step staining without secondary antibodies, simplifying multi-color panel design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw material sourcing, supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for integrin and cell adhesion research. Our portfolio extends to rare target raw-material procurement, supporting diagnostic manufacturers from early development through to clinical application. Contact us to streamline your diagnostic pipeline with reliable, high-quality reagents.