Rabbit anti-Human ITGA1/CD49a mAb for FC - P56199

Core Product Specifications and Parameters

Biological Background: ITGA1/CD49a Function and Localization

• ITGA1 encodes integrin alpha-1, also known as CD49a or VLA-1 (Very Late Antigen-1), a member of the integrin alpha chain family. It pairs with integrin beta-1 (CD29) to form the alpha1beta1 integrin heterodimer.
• Alpha1beta1 integrin functions as a receptor for laminin and collagen, recognizing the proline-hydroxylated sequence G-F-P-G-E-R in collagen, mediating cell adhesion to the extracellular matrix.
• The integrin is involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth, linking matrix adhesion to growth factor signaling pathways.
• ITGA1 is a transmembrane glycoprotein that undergoes post-translational modifications including N-glycosylation and disulfide bond formation, critical for its structural stability and ligand binding.
• The protein contains multiple calcium and magnesium binding sites, which are essential for integrin activation and ligand-binding affinity.
• Subcellularly, ITGA1 localizes to the plasma membrane as a single-pass type I membrane protein, facilitating outside-in and inside-out signaling.
• Key keywords associated with ITGA1 include cell adhesion, integrin, glycoprotein, metal-binding, and receptor, reflecting its role in cell-matrix interactions and signal transduction.
• Molecular weight is predicted at approximately 130.8 kDa; the mature glycosylated form migrates at around 131 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the extracellular domain of human ITGA1, suggesting the antibody likely recognizes the native conformation on live cells, suitable for flow cytometry applications.
• For FC, titrate the antibody to determine optimal concentration and include appropriate isotype controls to ensure specificity.
• Consider that ITGA1 is expressed on activated T cells, NK cells, and some epithelial cells. Validate the antibody staining in your target cell population using known positive and negative cell lines.
• Since the antibody is -conjugated, it is directly ready for flow cytometric analysis without the need for secondary staining. Protect from light and optimize compensation settings.
• Cross-reactivity is stated as human; for non-human species, verify sequence homology before use.

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