Rabbit anti-Human Integrin β5/ITGB5 Monoclonal Antibody for Flow Cytometry - P18084

Core Product Specifications and Parameters

Biological Background: Integrin beta-5 Function and Localization

• Integrin beta-5 (ITGB5) is a member of the integrin beta subunit family and forms heterodimers with alpha-V (ITGAV) to create the fibronectin receptor.
• The ITGAV:ITGB5 complex specifically recognizes the RGD (Arg-Gly-Asp) tripeptide motif in its ligand fibronectin, mediating cell adhesion.
• During microbial infection, ITGAV:ITGB5 serves as a host cell receptor for adenovirus type C, facilitating viral entry.
• Subcellularly, integrin beta-5 localizes to the cell membrane as a single-pass type I membrane protein.
• Post-translational modifications include glycosylation and phosphorylation, contributing to its functional regulation.
• Integrin beta-5 contains EGF-like domains and multiple disulfide bonds, which are critical for structural stability and ligand binding.
• The protein requires divalent cations (calcium and magnesium) for its adhesive functions.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal extracellular region (aa 24-490), making this antibody suitable for detecting cell surface integrin beta-5 by flow cytometry.
• For flow cytometry, use appropriate isotype controls and titrate the antibody to determine optimal staining concentration.
• This antibody is directly conjugated to , a bright red fluorescent dye, eliminating the need for a secondary antibody and reducing non-specific binding.
• Verify antibody specificity in your system by testing on known integrin beta-5 positive and negative cell lines.
• If working with non-human species, consider that cross-reactivity has not been confirmed; validate with the relevant species samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, including validated antibodies for adhesion and viral entry studies. We offer this anti-integrin beta-5 antibody alongside optimized antibody pairs, bulk ancillary reagents, and sourcing services for rare targets to streamline your assay development from concept to clinic. Leverage our expertise to secure high-quality reagents for your flow cytometry workflows.