Rabbit anti-Human Integrin β5/ITGB5 mAb for FC - P18084

Core Product Specifications and Parameters

Biological Background: Integrin β5/ITGB5 Function and Localization

• Integrin beta-5 (ITGB5) pairs with integrin alpha-V to form the integrin alpha-V/beta-5 heterodimer, a transmembrane receptor.
• ITGAV:ITGB5 functions as a receptor for fibronectin, recognizing the R-G-D sequence in its ligand, mediating cell adhesion.
• (Microbial infection) The integrin alpha-V/beta-5 complex acts as a receptor for adenovirus type C, facilitating host cell entry.
• ITGB5 localizes to the cell membrane and is expressed on the cell surface.
• The protein features an EGF-like domain, disulfide bonds, and calcium/magnesium-binding sites; it is a glycoprotein and phosphoprotein.
• The unprocessed precursor has a calculated molecular weight of approximately 88 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 24–490) spans the extracellular domain, making the antibody suitable for detecting surface ITGB5 by flow cytometry without permeabilization.
• Use a human cell line known to express ITGB5; include an isotype control and optimize antibody titration for your specific experiment.
• The conjugate allows direct detection; no secondary antibody is needed, but consider a blocking step to minimize non-specific binding.
• If studying viral entry, validate staining on adenovirus-infected cells to confirm receptor-specific detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to IVD raw materials, including this anti-Integrin β5 monoclonal antibody. For integrin-related research and assay development, we offer validated antibodies, recombinant proteins, and ancillary reagents in bulk, covering every stage from concept to clinic. Whether you need routine or rare target antibodies, our sourcing solutions bridge the gap between your project requirements and quality supply.