Rabbit anti-Human Integrin-β1/CD29 Monoclonal Antibody for FC - P05556

Core Product Specifications and Parameters

Biological Background: Integrin beta-1 Function and Localization

• Integrin beta-1 (ITGB1, CD29) is a ubiquitous cell adhesion receptor that heterodimerizes with at least 12 different alpha subunits, forming integrins that bind collagen, fibronectin, laminin, vitronectin, and other extracellular matrix components.
• The heterodimer ITGA5:ITGB1 acts as a receptor for fibronectin FN1 and mediates R-G-D-dependent cell adhesion to FN1. Related references:
  PMID:33962943
• ITGB1 serves as a signaling co‑receptor: ITGA4:ITGB1 binds fractalkine (CX3CL1) and participates in CX3CR1-dependent signaling; ITGA5:ITGB1 acts as a receptor for IL1B, essential for IL1B signaling; and ITGA5:ITGB3 binds soluble CD40LG, required for CD40/CD40LG signaling. Related references:
  PMID:23125415
  PMID:24789099
  PMID:29030430
  PMID:31331973
• The protein is localized to the cell membrane and enriched in adhesion structures: focal adhesions, invadopodia, lamellipodia, ruffles, and the cleavage furrow; it also traffics through recycling endosomes.
• ITGB1 is widely expressed across many tissues, with highest levels in placenta, smooth muscle, skin, liver, and various hematopoietic cells; alternative splicing generates isoforms with restricted expression, such as in striated muscle and peripheral T-cells.
• Several microbial pathogens exploit ITGB1 for host entry: it acts as a receptor for human echoviruses, cytomegalovirus, Epstein-Barr virus, parvovirus B19, rotavirus, reovirus, and HIV-1, and interacts with bacterial and fungal adhesins, including N. meningitidis nadA and mucormycosis-causing CotH proteins. Related references:
  PMID:32487760
• Post-translational modifications, including N-glycosylation, phosphorylation at multiple residues, acetylation, and ubiquitination, modulate ITGB1 stability, activation, and subcellular trafficking.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1-728 amino acids of the human ITGB1 extracellular domain, favoring recognition of surface-exposed epitopes; this makes the antibody well-suited for live-cell flow cytometry without permeabilization.
• For FC, titrate the conjugate to optimal concentration using your target cell type; include an isotype control and, if necessary, an Fc-receptor blocking step to minimize background.
• Because ITGB1 is highly expressed on most leukocytes and many adherent cells, confirm expression in your target population with a known positive control, such as Jurkat cells or THP-1 monocytes.
• Direct conjugation eliminates the need for a secondary antibody; ensure your cytometer is equipped with a 488 nm laser and appropriate emission filters (e.g., 530/30 nm) for detection of .
• For staining adherent cells, consider using gentle detachment (e.g., non-enzymatic disassociation buffer) to preserve surface integrin integrity, as trypsin may cleave the extracellular domain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutions with integrated access to premium IVD raw materials and technical expertise—from early development through clinical application. As your sourcing partner for integrin beta‑1 (CD29) reagents, we offer a comprehensive portfolio of validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing solutions for rare target raw materials. Streamline your cell adhesion, immunophenotyping, and oncology research with CamelBio’s efficient, end‑to‑end supply chain support.