Rabbit anti-Human Integrin-β1/CD29 Monoclonal Antibody for FC - P05556

Core Product Specifications and Parameters

Biological Background: Integrin beta-1 Function and Localization

• ITGB1 encodes integrin beta-1, a transmembrane receptor that pairs with multiple alpha subunits to form functional integrin heterodimers. Alternative splicing generates several isoforms; isoform 1 is ubiquitously expressed, while isoform 5 is restricted to striated muscles.
• Integrin beta-1 mediates cell adhesion to a wide range of extracellular matrix proteins, including collagen, fibronectin, laminin, vitronectin, and fibrinogen, thereby regulating cell spreading, migration, and tissue organization.
• Beyond classical ECM adhesion, ITGA4:ITGB1 and ITGA5:ITGB1 engage soluble ligands such as fractalkine (CX3CL1), PLA2G2A, IL1B, and CD40LG, as well as matrix components like fibrillin-1, to modulate intracellular signaling cascades. Related references: PMID:23125415, PMID:24789099, PMID:18635536, PMID:25398877, PMID:12807887, PMID:17158881, PMID:29030430, PMID:31331973
• ITGB1 localizes to the cell membrane, focal adhesions, invadopodia, lamellipodia, cleavage furrow, and recycling endosomes, highlighting its dynamic role in adhesion, migration, and cytokinesis.
• Post-translational modifications, including glycosylation, phosphorylation, disulfide bond formation, ubiquitination, and acetylation, regulate ITGB1 stability, trafficking, and function.
• The protein is a receptor for numerous pathogens: viruses such as HIV-1, cytomegalovirus, Epstein-Barr virus, and reovirus exploit ITGB1 for entry; additionally, Mucorales fungi utilize CotH-ITGB1 interactions for epithelial invasion. Related references: PMID:32487760
• Tissue expression is broad, with strong detection in placenta, vascular smooth muscle, skin, skeletal muscle, and liver. ITGB1 is essential for angiogenesis, osteoblast differentiation, myogenesis, and vascular tone regulation. Related references: PMID:1551917, PMID:7545396, PMID:7681433, PMID:33962943, PMID:35802072

Experimental Guidance and Technical Tips

• The conjugate allows direct detection via flow cytometry, eliminating the need for a secondary antibody.
• The immunogen comprises amino acids 1–728 (extracellular domain); live-cell staining without permeabilization may be possible. Verify with your cell system.
• Titration experiments (e.g., 0.1–5 µg per million cells) are recommended to determine the optimal staining concentration for your target cells.
• Use appropriate isotype controls and perform compensation when combining with other fluorophores.
• Fixation and permeabilization may alter epitope accessibility; validate any protocol changes in your assay.
• The antibody is specific for human integrin beta-1; cross-reactivity with other species has not been reported. Confirm reactivity experimentally if using non-human samples.
• Protect from light and store at 2–8°C; do not freeze.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with integrated access to premium IVD raw materials, including validated antibody pairs, conjugated monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our Rabbit anti-Human Integrin-β1/CD29 monoclonal antibody supports flow-cytometric profiling of integrin-mediated adhesion and signaling in immunology and oncology applications. Contact our team for tailored bulk supply and technical consultation to accelerate your assay development.