Rabbit anti-Human Integrin alpha 3/CD49c mAb - P26006

Core Product Specifications and Parameters

Biological Background: Integrin alpha-3 Function and Localization

• Integrin alpha-3 (CD49c, VLA-3a) is a transmembrane glycoprotein that non-covalently pairs with integrin beta-1 (ITGB1) to form the VLA-3 heterodimeric adhesion receptor.
• ITGA3:ITGB1 serves as a receptor for multiple extracellular matrix proteins including fibronectin, laminin, collagen, epiligrin, thrombospondin, and CSPG4, mediating cell adhesion and migration.
• The receptor provides a docking site for fibroblast activation protein (FAP) at invadopodia plasma membranes in a collagen-dependent manner, facilitating matrix degradation and promoting cellular invasion.
• In endothelial cells, alpha-3/beta-1 associates with LGALS3 to mediate CSPG4-stimulated cell migration.
• During microbial infection, ITGA3:ITGB1 functions as a receptor for R.delemar CotH7 on alveolar epithelial cells, potentially representing an early step in pulmonary mucormycosis.
• Integrin alpha-3 localizes to the cell membrane and is enriched at specialized membrane structures including invadopodium and filopodium membranes.
• Two isoforms generated by alternative splicing exhibit distinct tissue expression: isoform 1 is widely expressed, while isoform 2 is restricted to brain and heart. In the heart, isoform 2 is specific to endothelial vein cells, whereas isoform 1 is found on vascular smooth muscle cells.
• The protein undergoes several post-translational modifications, including phosphorylation, palmitoylation, and proteolytic cleavage at paired basic residues, which regulate its function and trafficking.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration using a known positive human cell line (e.g., A549, MDA-MB-231) or primary cells.
• This antibody is directly conjugated to ; ensure appropriate compensation and filter settings (excitation ~650 nm, emission ~665 nm) for detection.
• The immunogen targets an extracellular domain (aa 466–620); therefore, the antibody is predicted to recognize native surface-expressed integrin alpha-3. Validate in the relevant sample system.
• When staining adherent cells, consider gentle detachment methods (e.g., non-enzymatic cell dissociation buffer) to preserve surface antigen integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is committed to supporting diagnostic manufacturers and researchers with comprehensive IVD raw material solutions. For integrin biology and cell adhesion research, we offer validated antibody pairs, optimized monoclonal antibodies like this anti-CD49c clone, and bulk ancillary reagents to streamline your assay development. Our sourcing capabilities extend to rare targets, enabling breakthroughs from concept to clinic.