Rabbit anti-Human ILT5/CD85a Monoclonal Antibody for Flow Cytometry - O75022

Core Product Specifications and Parameters

Biological Background: ILT5/CD85a (LILRB3) Function and Localization

• ILT5/CD85a, encoded by the LILRB3 gene, is also known as leukocyte immunoglobulin-like receptor subfamily B member 3, CD85 antigen-like family member A, immunoglobulin-like transcript 5 (ILT-5), and monocyte inhibitory receptor HL9.
• It may function as a receptor for class I MHC antigens and becomes activated upon co-ligation with immune receptors such as FCGR2B and the B-cell receptor.
• Activated LILRB3 down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIMs).
• The receptor belongs to the leukocyte immunoglobulin-like receptor (LILR) family, which includes both activating and inhibitory members.
• LILRB3 is a cell membrane protein with a signal peptide, extracellular immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail containing ITIMs.
• It undergoes alternative splicing and post-translational modifications, including glycosylation and phosphorylation.
• Tissue expression is primarily detected in monocytes and B-cells, consistent with its role in innate and adaptive immune regulation.

Experimental Guidance and Technical Tips

• The recombinant immunogen spans the extracellular region (aa 24–418), covering all immunoglobulin-like domains, likely yielding antibodies recognizing native surface LILRB3.
• For flow cytometry, titrate the antibody on human peripheral blood mononuclear cells (PBMCs) or isolated B-cells/monocytes, and include Fc receptor blocking to reduce non-specific binding.
• Consider using isotype controls and fluorescence-minus-one (FMO) controls to confirm specificity in multicolor panels.
• Since LILRB3 is an inhibitory receptor, co-staining with activation markers can help assess its expression dynamics in immune cell subsets.
• Keep in mind that LILRB3 may be co-expressed with other LILR family members; verify specificity using appropriate knockouts or blocking peptides where necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a streamlined sourcing pipeline for IVD raw materials. This -conjugated anti-ILT5/CD85a antibody supports flow cytometry assays in immunology and immune-oncology research. Beyond conjugated primary antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing for rare targets, helping accelerate your assay development from concept to clinic.